LOCUS       sequence-85691-e        1555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      797..806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             803..1513
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcgcggc
       61 tgcacgctcc tcgggacaca gtgccaacca tccagaggac aagatggatt ggggcacact
      121 acagagcatc ctcgggggtg tcaacaagca ctccaccagc attgggaaaa tctggctcac
      181 tgtcctcttc atcttccgca tcatgatcct cgtggtggcc gcgaaggagg tgtggggaga
      241 tgagcaagcc gattttgttt gcaacactct ccagcctggc tgtaagaatg tgtgctacga
      301 ccactacttc cccatctctc acatccggct ctgggctctg cagctgatca tggtgtccac
      361 gccggccctc ctggtagcta tgcacgtggc ctaccggaga cacgaaaaga aacggaagtt
      421 catgaaggga gagataaaga acgagtttaa ggacatcgaa gagatcaaaa cccagaaggt
      481 ccgtatcgaa gggtccctgt ggtggaccta caccaccagc atcttcttcc gggtcatctt
      541 cgaagctgtc ttcatgtatg tcttttacat catgtacaat ggcttcttca tgcagcgtct
      601 ggtgaagtgt aacgcctggc cttgtcccaa tacagtggac tgcttcattt ccaggcccac
      661 agaaaagact gtcttcacgg tgttcatgat ctctgtgtct ggaatttgca tcctgctaaa
      721 catcacagag ctgtgctatc tgttcattag gtattgctca gggaagtcca aaagaccagt
      781 cgcggatcca ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat
      841 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
      901 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
      961 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     1021 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     1081 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     1141 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     1201 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
     1261 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     1321 tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     1381 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     1441 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     1501 gcacaagctt aattaagcgg ccgcgactct agatcataat cagccatacc acatt
//
