LOCUS       sequence-85693-e        2166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1407..1416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1413..2123
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcggca
       61 gcacttttct ttcattgggg ggaaggcgtg aggaaagtac caaacagcag cagactttta
      121 aactttaaac agacaggtct gagagcctga actctcattt ttcctttgac ttcagcctcc
      181 aaggagttcc accaactttg gcgcgccggc ttcactttca ttaagtgaaa gagaggtgcc
      241 cagacatggg tgactggagt gccttgggga agcttctgga caaggtccaa gcctactcca
      301 ccgctggagg gaaggtgtgg ctgtcagtgc tcttcatatt cagaatcctg ctcctgggga
      361 cagctgttga gtcagcttgg ggtgatgaac agtctgcctt tcgctgtaac actcaacaac
      421 ctggctgcga aaacgtctgc tatgacaagt ccttccccat ctctcacgtg cgcttctggg
      481 tccttcagat catattcgtg tctgtgccca cactcctgta cttggcccat gtgttctatg
      541 tgatgaggaa ggaagagaag ctaaacaaga aagaagagga gctcaaagtg gcccagactg
      601 acggggtcaa cgtggagatg cacctgaagc agattgaaat caagaagttc aagtacggga
      661 ttgaagagca cggcaaggtg aaaatgaggg gcggcttgct gagaacctac atcatcagca
      721 tcctcttcaa gtctgtcttc gaggtggcct tcctgctcat ccagtggtac atctatgggt
      781 tcagcttgag cgcggtctac acctgcaaga gagatccctg cccccaccag gtagactgct
      841 tcctctcacg tcccacggag aaaaccatct tcatcatctt catgctggtg gtgtccttgg
      901 tgtctctcgc tttgaacatc attgagctct tctacgtctt cttcaaaggc gttaaggatc
      961 gcgtgaaggg aagaagcgat ccttaccacg ccaccactgg cccactgagc ccatcaaaag
     1021 actgcggatc tccaaaatac gcctacttca atggctgctc ctcaccaacg gctccactct
     1081 cgcctatgtc tcctcctggg tacaagctgg ttactggtga cagaaacaat tcctcgtgcc
     1141 gcaattacaa caagcaagct agcgagcaaa actgggcgaa ctacagcgca gagcaaaatc
     1201 gcatggggca ggccggaagc accatctcca actcgcacgc ccagccgttc gatttccccg
     1261 acgacaacca gaatgccaaa aaagttgctg ctggacatga actccagcca ttagccatcg
     1321 tggaccaacg accttccagc agagccagca gccgcgccag cagcaggcct cggcctgatg
     1381 acctggagat tgcggatcca ccggtcgcca ccatggtgtc taagggcgaa gagctgatta
     1441 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     1501 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     1561 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     1621 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     1681 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     1741 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     1801 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     1861 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     1921 gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga
     1981 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     2041 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     2101 gcaaactggg gcacaagctt aattaagcgg ccgcgactct agatcataat cagccatacc
     2161 acattt
//
