LOCUS       sequence-85694-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      241..250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             247..957
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca
       61 gatctggtac cccttgcagc ttccagttgt caggaagaga aggctcccat ggatcctgtg
      121 gtggtgctgg ggctctgtct ctcctgtttg cttctccttt cactctggaa acagagctat
      181 gggggaggga agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
      241 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
      301 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
      361 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      421 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
      481 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
      541 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      601 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
      661 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
      721 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
      781 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
      841 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
      901 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa
      961 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1021 aaaaaacctc ccacactccc cctgaacctg aaacataaat gaatg
//
