LOCUS       sequence-85696-e        1543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             790..1500
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 agatccgcta gcgccaccat ggaatatcat cctgatttag aaaatttgga tgaagatgga
       61 tatactcaat tacacttcga ctctcaaagc aataccagga tagctgttgt ttcagagaaa
      121 ggatcgtgtg ctgcatctcc tccttggcgc ctcattgctg taattttggg aatcctatgc
      181 ttggtaatac tggtgatagc tgtggtcctg ggtaccatgg ctatttggag atccaattca
      241 ggaagcaaca cattggagaa tggctacttt ctatcaagaa ataaagagaa ccacagtcaa
      301 cccacacaat catctttaga agacagtgtg actcctacca aagctgtcaa aaccacaggg
      361 gttctttcca gcccttgtcc tcctaattgg attatatatg agaagagctg ttatctattc
      421 agcatgtcac taaattcctg ggatggaagt aaaagacaat gctggcaact gggctctaat
      481 ctcctaaaga tagacagctc aaatgaattg ggatttatag taaaacaagt gtcttcccaa
      541 cctgataatt cattttggat aggcctttct cggccccaga ctgaagtacc atggctctgg
      601 gaggatggat caacattctc ttctaactta tttcagatca gaaccacagc tacccaagaa
      661 aacccatctc caaattgtgt atggattcac gtgtcagtca tttatgacca actgtgtagt
      721 gtgccctcat atagtatttg tgagaagaag ttttcaatga gctccggagg ggatccaccg
      781 gtcgccacca tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg
      841 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
      901 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
      961 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     1021 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     1081 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     1141 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     1201 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     1261 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     1321 gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     1381 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     1441 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     1501 taagcggccg cgactctaga tcataatcag ccataccaca ttt
//
