LOCUS       sequence-85698-e        1480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Endosomes-14
FEATURES             Location/Qualifiers
     source          1..1480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..745
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             791..820
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg tctaagggcg aagagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      241 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      481 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      541 gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa
      601 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      661 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaagc ttaattccgg atcagatctc gagctcaagc ttgatatcga
      781 attccccatg gagcagaagc tgatctccga ggaggacctg aaccggaaga aactggtggt
      841 tgttggtgat ggagcctgtg gaaagacatg cttgctcata gtcttcagca aggacgagtt
      901 cccagaggtg tatgtgccca cagtgtttga gaactatgtg gcagatatcg aggtggatgg
      961 aaagcaggta gagttggctt tgtgggacac agctggccag gaggactacg accgcctgcg
     1021 gccgctctcc tacccggaca ccgacgtcat tctcatgtgc ttctcggtgg acagcccgga
     1081 ctcgctggag aacatccccg agaagtgggt ccccgaggtg aagcacttct gtcccaatgt
     1141 gcccatcatc ctggtggcca acaaaaaaga cctgcgcagc gacgagcatg tccgcacaga
     1201 gctggcccgc atgaagcagg aacccgttcg cacggatgac ggccgcgcca tggccgtgcg
     1261 catccaagcc tacgactacc tcgagtgctc tgccaagacc aaggaaggcg tgcgcgaggt
     1321 cttcgagacg gccacgcgcg ccgcgctgca gaagcgctac ggctcccaga acggctgcat
     1381 caactgctgc aaggtgctat gagggccgcg cccgtcgcgc ctgcccctgc cggggatcca
     1441 ccggatctag ataactgatc ataatcagcc ataccacatt
//
