LOCUS       sequence-85699-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-ER-5
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..801
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acggtcagat ccgctagcgc caccatgctg ctatccgtgc cgttgctgct cggcctcctc
       61 ggcctggccg tcgccgcacc ggtcgccacc atggtgtcta agggcgaaga gctgattaag
      121 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
      181 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
      241 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
      301 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
      361 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
      421 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
      481 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
      541 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
      601 gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac
      661 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
      721 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
      781 aaactggggc acaagcttaa ttccggaaag gacgagctgt aagaattctg cagtcgacgg
      841 taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
