LOCUS       sequence-85700-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..787
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
       61 gctaccggtc gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat
      121 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
      181 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
      241 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
      301 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
      361 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
      421 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
      481 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
      541 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
      601 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
      661 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
      721 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
      781 gcttaattcc ggactcagat ctaagctgaa ccctcctgat gagagtggcc ccggctgcat
      841 gagctgcaag tgtgtgctct cctgagaatt ctgcagtcga cggtaccgcg ggcccgggat
      901 ccaccggatc tagataactg atcataatca gccataccac att
//
