LOCUS       sequence-85701-e        1771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..738
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(1534..1545)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcggt ggccaccatg gtgtctaagg gcgaagagct gattaaggag
       61 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
      121 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
      181 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
      241 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
      361 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
      481 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
      541 agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc
      601 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
      661 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca agcttaattc cggactcaga tctcgagcca tgaagccagg attcagtccc
      781 cgtgggggtg gctttggcgg ccgagggggc tttggtgacc gtggtggtcg tggaggccga
      841 gggggctttg gcgggggccg aggccaaggc ggaggcttta gaggtcgtgg acgaggagga
      901 ggtggaggcg gcggcggcgg tggaggagga ggaagaggtg gtggaggctt ccattttggt
      961 ggcaaccggg gtcgtggtcg gggaggaaaa agaggaaacc agtcggggaa gaatgtgatg
     1021 gtggagccgc atcggcatga gggtgtcttc atttgtcggg gaaaggaaga tgcactggtc
     1081 accaagaacc tggtccctgg ggaatcagtt tatggagaga agagagtctc gatttcggaa
     1141 ggagatgaca aaattgagta ccgagcctgg aaccccttcc gctccaagct tgcagcagca
     1201 atcctgggtg gtgtggacca gatccacatc aaaccggggg ctaaggttct ctacctcggg
     1261 gctgcctcgg gcaccacggt ctcccatgtc tctgacatcg ttggtccgga tggtctagtc
     1321 tatgcagtcg agttctccca ccgctctggc cgtgacctca ttaacttggc caagaagagg
     1381 accaacatca ttcctgtgat cgaggatgct cgacacccac acaaataccg catgctcatc
     1441 gcaatggtgg atgtgatctt tgctgatgtg gcccagccag accagacccg gattgtggcc
     1501 ctgaatgccc acaccttcct gcgtaatgga ggacactttg tgatttccat taaggccaac
     1561 tgcattgact ccacagcctc agccgaggcc gtgtttgcct ccgaagtgaa aaagatgcaa
     1621 caggagaaca tgaagccgca ggagcagttg acccttgagc catatgaaag agaccatgcc
     1681 gtggtcgtgg gagtgtacag gccacccccc aaggtgaaga actgaggatc caccggatct
     1741 agataactga tcataatcag ccataccaca t
//
