LOCUS       sequence-85707-e        1261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      502..511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             508..1218
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gaacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgccaccatg
       61 gcccgaacca agcagaccgc taggaagtcc accggtggga aagccccccg caaacagctg
      121 gccaccaagg cggctcggaa aagcgcgccc tctaccggcg gggtgaagaa gcctcaccgc
      181 tacaggccag ggaccgtggc tctgagagag atccgtcgtt accagaaatc gactgagctg
      241 ctcatccgga agctgccatt ccagagattg gtgagggaga tcgcccagga tttcaaaacc
      301 gacttgaggt ttcaaagtgc agccatcggt gcccttcagg aggctagcga agcatacctg
      361 gtggggttgt ttgaagatac caatctgtgt gccatccacg ccaagagagt caccatcatg
      421 cccaaagaca tccagttggc tcgccggata cggggggaga gagctgggtc cggaagcgca
      481 ggcagtgggg atccaccggt cgccaccatg gtgtctaagg gcgaagagct gattaaggag
      541 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
      601 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
      661 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
      721 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
      781 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
      841 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
      901 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
      961 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     1021 agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc
     1081 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     1141 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     1201 ctggggcaca agcttaatta agcggccgcg actctagatc ataatcagcc ataccacatt
     1261 t
//
