LOCUS       sequence-85708-e        3241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 399, Emission: 
            456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..3241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2483..2492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2489..3199
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagaccgcc atgaatttac aaccaatttt
       61 ctggattgga ctgatcagtt cagtttgctg tgtgtttgct caaacagatg aaaatagatg
      121 tttaaaagca aatgccaaat catgtggaga atgtatacaa gcagggccaa attgtgggtg
      181 gtgcacaaat tcaacatttt tacaggaagg aatgcctact tctgcacgat gtgatgattt
      241 agaagcctta aaaaagaagg gttgccctcc agatgacata gaaaatccca gaggctccaa
      301 agatataaag aaaaataaaa atgtaaccaa ccgtagcaaa ggaacagcag agaagctcaa
      361 gccagaggat attactcaga tccaaccaca gcagttggtt ttgcgattaa gatcagggga
      421 gccacagaca tttacattaa aattcaagag agctgaagac tatcccattg acctctacta
      481 ccttatggac ctgtcttact caatgaaaga cgatttggag aatgtaaaaa gtcttggaac
      541 agatctgatg aatgaaatga ggaggattac ttcggacttc agaattggat ttggctcatt
      601 tgtggaaaag actgtgatgc cttacattag cacaacacca gctaagctca ggaacccttg
      661 cacaagtgaa cagaactgca ccagcccatt tagctacaaa aatgtgctca gtcttactaa
      721 taaaggagaa gtatttaatg aacttgttgg aaaacagcgc atatctggaa atttggattc
      781 tccagaaggt ggtttcgatg ccatcatgca agttgcagtt tgtggatcac tgattggctg
      841 gaggaatgtt acacggctgc tggtgttttc cacagatgcc gggtttcact ttgctggaga
      901 tgggaaactt ggtggcattg ttttaccaaa tgatggacaa tgtcacctgg aaaataatat
      961 gtacacaatg agccattatt atgattatcc ttctattgct caccttgtcc agaaactgag
     1021 tgaaaataat attcagacaa tttttgcagt tactgaagaa tttcagcctg tttacaagga
     1081 gctgaaaaac ttgatcccta agtcagcagt aggaacatta tctgcaaatt ctagcaatgt
     1141 aattcagttg atcattgatg catacaattc cctttcctca gaagtcattt tggaaaacgg
     1201 caaattgtca gaaggagtaa caataagtta caaatcttac tgcaagaacg gggtgaatgg
     1261 aacaggggaa aatggaagaa aatgttccaa tatttccatt ggagatgagg ttcaatttga
     1321 aattagcata acttcaaata agtgtccaaa aaaggattct gacagcttta aaattaggcc
     1381 tctgggcttt acggaggaag tagaggttat tcttcagtac atctgtgaat gtgaatgcca
     1441 aagcgaaggc atccctgaaa gtcccaagtg tcatgaagga aatgggacat ttgagtgtgg
     1501 cgcgtgcagg tgcaatgaag ggcgtgttgg tagacattgt gaatgcagca cagatgaagt
     1561 taacagtgaa gacatggatg cttactgcag gaaagaaaac agttcagaaa tctgcagtaa
     1621 caatggagag tgcgtctgcg gacagtgtgt ttgtaggaag agggataata caaatgaaat
     1681 ttattctggc aaattctgcg agtgtgataa tttcaactgt gatagatcca atggcttaat
     1741 ttgtggagga aatggtgttt gcaagtgtcg tgtgtgtgag tgcaacccca actacactgg
     1801 cagtgcatgt gactgttctt tggatactag tacttgtgaa gccagcaacg gacagatctg
     1861 caatggccgg ggcatctgcg agtgtggtgt ctgtaagtgt acagatccga agtttcaagg
     1921 gcaaacgtgt gagatgtgtc agacctgcct tggtgtctgt gctgagcata aagaatgtgt
     1981 tcagtgcaga gccttcaata aaggagaaaa gaaagacaca tgcacacagg aatgttccta
     2041 ttttaacatt accaaggtag aaagtcggga caaattaccc cagccggtcc aacctgatcc
     2101 tgtgtcccat tgtaaggaga aggatgttga cgactgttgg ttctatttta cgtattcagt
     2161 gaatgggaac aacgaggtca tggttcatgt tgtggagaat ccagagtgtc ccactggtcc
     2221 agacatcatt ccaattgtag ctggtgtggt tgctggaatt gttcttattg gccttgcatt
     2281 actgctgata tggaagcttt taatgataat tcatgacaga agggagtttg ctaaatttga
     2341 aaaggagaaa atgaatgcca aatgggacac gggtgaaaat cctatttata agagtgccgt
     2401 aacaactgtg gtcaatccga agtatgaggg aaaaggtagc gcatccgggg gtgcgtcagg
     2461 cggcagcgga tctggaccgg tcgccaccat ggtgtctaag ggcgaagagc tgattaagga
     2521 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     2581 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     2641 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     2701 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     2761 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     2821 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     2881 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     2941 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     3001 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     3061 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     3121 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     3181 actggggcac aagcttaatt aagcggccgc gactctagat cataatcagc cataccacat
     3241 t
//
