LOCUS       sequence-85714-e        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             393..1103
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgacgtcag atccgctagc gccaccatga agttaagtcg ccagttcacc gtgtttggca
       61 gcgcgatctt ctgcgtcgta atcttctcac tctacctgat gctggacagg ggtcacttgg
      121 actaccctcg gggcccgcgc caggagggct cctttccgca gggccagctt tcaatattgc
      181 aagaaaagat tgaccatttg gagcgtttgc tcgctgagaa caacgagatc atctcaaata
      241 tcagagactc agtcatcaac ctgagcgagt ctgtggagga cggcccgcgg gggtcaccag
      301 gcaacgccag ccaaggctcc atccacctcc actcgccaca gttggccctg caggctgacc
      361 ccagctccgg atcggatcca ccggtcgcca ccatggtgtc taagggcgaa gagctgatta
      421 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
      481 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
      541 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
      601 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
      661 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      721 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
      781 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
      841 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
      901 gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga
      961 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     1021 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     1081 gcaaactggg gcacaagctt aattaagcgg ccgcgactct agatcataat cagccatacc
     1141 acatt
//
