LOCUS       sequence-85716-e        1880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..747
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgtctaaggg cgaagagctg
       61 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
      121 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      181 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
      241 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
      301 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      361 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
      421 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
      481 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
      541 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
      601 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
      661 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
      721 cctagcaaac tggggcacaa gcttaattcc ggaagcggcg ggtccgggag cggaagcgct
      781 gagccccgcc aggagttcga agtgatggaa gatcacgctg ggacgtacgg gttgggggac
      841 aggaaagatc aggggggcta caccatgcac caagaccaag agggtgacac ggacgctggc
      901 ctgaaagctg aagaagcagg cattggagac acccccagcc tggaagacga agctgctggt
      961 cacgtgaccc aagctcgcat ggtcagtaaa agcaaagacg ggactggaag cgatgacaaa
     1021 aaagccaagg gggctgatgg taaaacgaag atcgccacac cgcggggagc agcccctcca
     1081 ggccagaagg gccaggccaa cgccaccagg attccagcaa aaaccccgcc cgctccaaag
     1141 acaccaccca gctctggtga acctccaaaa tcaggggatc gcagcggcta cagcagcccc
     1201 ggctccccag gcactcccgg cagccgctcc cgcaccccgt cccttccaac cccacccacc
     1261 cgggagccca agaaggtggc agtggtccgt actccaccca agtcgccgtc ttccgccaag
     1321 agccgcctgc agacagcccc cgtgcccatg ccagacctga agaatgtcaa gtccaagatc
     1381 ggctccactg agaacctgaa gcaccagccg ggaggcggga aggtgcaaat agtctacaaa
     1441 ccagttgacc tgagcaaggt gacctccaag tgtggctcat taggcaacat ccatcataaa
     1501 ccaggaggtg gccaggtgga agtaaaatct gagaagcttg acttcaagga cagagtccag
     1561 tcgaagattg ggtccctgga caatatcacc cacgtccctg gcggaggaaa taaaaagatt
     1621 gaaacccaca agctgacctt ccgcgagaac gccaaagcca agacagacca cggggcggag
     1681 atcgtgtaca agtcgccagt ggtgtctggg gacacgtctc cacggcatct cagcaatgtc
     1741 tcctccaccg gcagcatcga catggtagac tcgccccagc tcgccacgct agctgacgag
     1801 gtgtctgcct ccctggccaa gcagggtttg tgaggatcca ccggatctag ataactgatc
     1861 ataatcagcc ataccacatt
//
