LOCUS       sequence-85717-e        1876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1123..1833
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag gccaccatgg ctgagccccg ccaggagttc
       61 gaagtgatgg aagatcacgc tgggacgtac gggttggggg acaggaaaga tcaggggggc
      121 tacaccatgc accaagacca agagggtgac acggacgctg gcctgaaagc tgaagaagca
      181 ggcattggag acacccccag cctggaagac gaagctgctg gtcacgtgac ccaagctcgc
      241 atggtcagta aaagcaaaga cgggactgga agcgatgaca aaaaagccaa gggggctgat
      301 ggtaaaacga agatcgccac accgcgggga gcagcccctc caggccagaa gggccaggcc
      361 aacgccacca ggattccagc aaaaaccccg cccgctccaa agacaccacc cagctctggt
      421 gaacctccaa aatcagggga tcgcagcggc tacagcagcc ccggctcccc aggcactccc
      481 ggcagccgct cccgcacccc gtcccttcca accccaccca cccgggagcc caagaaggtg
      541 gcagtggtcc gtactccacc caagtcgccg tcttccgcca agagccgcct gcagacagcc
      601 cccgtgccca tgccagacct gaagaatgtc aagtccaaga tcggctccac tgagaacctg
      661 aagcaccagc cgggaggcgg gaaggtgcaa atagtctaca aaccagttga cctgagcaag
      721 gtgacctcca agtgtggctc attaggcaac atccatcata aaccaggagg tggccaggtg
      781 gaagtaaaat ctgagaagct tgacttcaag gacagagtcc agtcgaagat tgggtccctg
      841 gacaatatca cccacgtccc tggcggagga aataaaaaga ttgaaaccca caagctgacc
      901 ttccgcgaga acgccaaagc caagacagac cacggggcgg agatcgtgta caagtcgcca
      961 gtggtgtctg gggacacgtc tccacggcat ctcagcaatg tctcctccac cggcagcatc
     1021 gacatggtag actcgcccca gctcgccacg ctagctgacg aggtgtctgc ctccctggcc
     1081 aagcagggtt tgagctccgg aggggatcca ccggtcgcca ccatggtgtc taagggcgaa
     1141 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     1201 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     1261 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     1321 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     1381 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     1441 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     1501 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     1561 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     1621 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     1681 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     1741 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     1801 gacctcccta gcaaactggg gcacaagctt aattaagcgg ccgcgactct agatcataat
     1861 cagccatacc acattt
//
