LOCUS       sequence-85720-e        6757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..748
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgtctaagg gcgaagagct
       61 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
      121 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
      181 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
      241 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
      301 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
      361 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
      421 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
      481 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
      541 cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc
      601 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
      661 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
      721 ccctagcaaa ctggggcaca agcttaattc cggactcaga tctggcagcg gtggaggcag
      781 cgcatccggc ggaagcggaa gcgctcagca ggctgcagac aagtacctct atgtggataa
      841 aaacttcatc aataacccgc tggcccaagc tgactgggct gccaagaagt tggtatgggt
      901 gccttccagc aagaatggct ttgaaccagc cagcctcaag gaggaggtgg gagaagaggc
      961 cattgtagag ctggtagaga atgggaagaa ggtgaaggtg aacaaggacg acatccagaa
     1021 gatgaaccca cccaagttct ccaaggtgga ggacatggca gagctcacgt gcctcaacga
     1081 agcttcggtg ctgcacaacc tcaaggagcg atactactca gggcttatct acacctattc
     1141 aggcctgttc tgtgtggtca tcaaccctta taagaacctg cccatctact cagaggagat
     1201 cgtggagatg tacaagggca agaagaggca cgagatgcca ccccacatct acgccatcac
     1261 agatactgcc taccggagca tgatgcagga ccgggaagat cagtccatcc tgtgcacggg
     1321 ggagtctgga gcagggaaga cagagaacac caagaaagtc atccagtacc tggcacatgt
     1381 ggcctcctca cacaagagca agaaggacca gggggagttg gagcggcagc tgctacaggc
     1441 caaccctatc ctagaggcct ttggaaacgc caagacggtg aagaatgaca actcctctcg
     1501 attcggtaaa ttcattcgta tcaactttga tgtcaatggc tacattgttg gtgccaacat
     1561 tgagacttat cttctggaga aatctcgtgc tatccgccaa gccaaagagg agcggacctt
     1621 ccacatcttc tactacctgc tgtctggggc cggagaacac ctgaagactg atctcctgtt
     1681 ggagccatac aacaaatacc gcttcctgtc caacgggcac gtcaccatcc ctgggcagca
     1741 ggacaaggac atgttccagg agacaatgga ggccatgaga attatgggta tcccagagga
     1801 tgagcagatg ggcttgctgc gggtcatctc tggggtcctt cagcttggca acattgcctt
     1861 caagaaggag cggaacactg accaggcgtc catgccggac aacacagctg ctcaaaaggt
     1921 gtcccacctc ctggggatca atgtgaccga cttcaccaga ggcatcctca ccccacgcat
     1981 caaggtgggc agagactatg tgcagaaggc gcagactaaa gagcaggctg actttgccat
     2041 tgaggccttg gccaaggcta cctatgagcg gatgttccgc tggctggtgc ttcgcatcaa
     2101 caaagctctg gacaagacca agaggcaggg cgcctcattt atcgggatac tggacatcgc
     2161 tggctttgaa atctttgatc tgaactcctt cgagcagctg tgcatcaact acaccaacga
     2221 gaagctgcag cagctgttca accacaccat gttcatcctg gagcaggagg agtaccagcg
     2281 agagggcatc gagtggaact tcatcgactt cggcctggac ctgcagccct gcatcgacct
     2341 cattgagaag ccggcgggtc ccccaggcat cctggccctg ctagatgagg agtgctggtt
     2401 tcctaaggcc actgacaaga gcttcgtgga gaaggtggtg caggagcagg gcacccaccc
     2461 caagttccag aagcccaagc aactgaagga caaggctgat ttctgcatta tccactatgc
     2521 cggcaaggtg gactataaag ctgacgagtg gctgatgaag aacatggacc ccttgaacga
     2581 caacatcgcc acgctgcttc accagtcctc agacaagttt gtctctgagc tgtggaagga
     2641 tgtggatcgg atcattggct tggaccaagt ggctggaatg tccgagacag cactacctgg
     2701 tgccttcaag acccggaagg gcatgttccg tactgtcgga cagctgtaca aggagcagct
     2761 ggccaagctc atggccacgt tgaggaatac caaccccaac ttcgtgcgct gcatcattcc
     2821 caaccatgag aagaaggccg gcaaactgga cccgcacttg gtgctggacc agctgcgctg
     2881 caatggcgtc cttgagggca tccggatctg ccgccagggc tttcccaaca gggtggtctt
     2941 ccaggagttc cggcagaggt atgagatcct cacccccaac tccatcccga agggcttcat
     3001 ggatggcaag caagcgtgtg tgctcatgat caaagccttg gagcttgaca gcaacctgta
     3061 ccgcatcggc cagagcaaag tgttcttccg ggcaggagtg ctcgcccacc tggaggaaga
     3121 gcgggacctg aagatcaccg atgtcatcat tggcttccag gcctgctgca ggggctacct
     3181 ggccaggaag gcctttgcca agaggcagca acagctgacc gccatgaagg tcctacagag
     3241 gaactgtgct gcgtacctca ggctgcgcaa ctggcagtgg tggaggctct tcaccaaggt
     3301 caagcccctg ttgaactcaa taagacatga ggatgagctg ttagccaagg aggcggaact
     3361 gacaaaggtt cgagagaaac atctggctgc agagaacagg ctgacagaga tggagacgat
     3421 gcagtctcag ctcatggcag agaagctgca gcttcaggag cagctgcagg cggagacaga
     3481 gctgtgtgcc gaggctgagg agctccgggc ccgtctgaca gcgaagaagc aggagctgga
     3541 ggagatatgc catgacctgg aggccagggt ggaggaggag gaggagcgct gccagtacct
     3601 gcaggccgag aagaagaaga tgcagcagaa catccaggaa cttgaggagc agttggagga
     3661 ggaggagagc gcccggcaga agctgcagct tgagaaggtg accaccgagg ccaagctgaa
     3721 gaaactggag gaggaccaga tcatcatgga ggaccagaac tgcaaactgg ccaaggagaa
     3781 gaaactgctg gaagacagag tagctgagtt cactaccaac ctcatggaag aggaggagaa
     3841 gtccaagagc ctggccaagc tcaagaacaa gcacgaggca atgatcaccg acctggaaga
     3901 gcgcctccgt agggaggaga agcagaggca ggagttggag aagacccgtc gcaagctgga
     3961 gggagactcc acagacctca gtgaccagat tgctgagctc caggcgcaga tagcagagct
     4021 caagatgcag ctggccaaga aggaggagga gttgcaggct gccttggcca gagtggaaga
     4081 agaagctgct cagaagaata tggccctgaa gaagatccga gaactggaaa ctcagatatc
     4141 tgagctccag gaggacctgg agtcggagcg agcctccagg aataaagccg agaagcagaa
     4201 acgggatctg ggagaggagc tggaggcgct gaagacagag ctggaggaca cgctggactc
     4261 cacggctgcc cagcaggagc tgaggtcgaa gcgtgagcag gaggtgagca tcctgaagaa
     4321 gactctggag gacgaggcca agacccatga ggcccagatc caggagatga ggcagaagca
     4381 ctcacaggct gtggaggagc tggcagatca gttggagcag acgaagcggg taaaagctac
     4441 ccttgagaag gcgaagcaga ccctggagaa tgagcgggga gagctggcca atgaggtgaa
     4501 ggccctgctg caaggcaagg gcgactcaga gcacaagcgc aagaaggtgg aggcgcagct
     4561 gcaagaactg caggtcaagt tcagcgaggg agagcgcgtg cgaaccgaac tggccgacaa
     4621 ggtcaccaag ctgcaggttg aactggacag tgtgaccggt ctccttagcc agtctgacag
     4681 caagtccagc aagcttacga aggacttctc tgcgctggag tcccagcttc aggacacaca
     4741 ggagttgctc caggaggaga accggcagaa gctgagcctg agcaccaagc tcaagcagat
     4801 ggaggatgag aaaaactcct tcagggagca gctggaggag gaggaggagg ccaagcgcaa
     4861 cttggagaag cagatcgcca cgctccatgc ccaggtgacc gacatgaaga agaagatgga
     4921 ggacggtgta gggtgcctgg agactgcaga ggaggcgaag cggaggcttc agaaggactt
     4981 ggaaggcctg agccagcggc ttgaggagaa ggtggctgcc tacgataagc tggagaagac
     5041 caagacacgg ctgcagcagg agctggacga cctgctggtt gacctggacc accagcggca
     5101 gagcgtctcc aacctggaaa agaagcagaa gaagttcgac cagctcctag ccgaggagaa
     5161 gaccatctcg gccaagtatg cagaggagcg tgaccgagct gaggctgagg cccgtgagaa
     5221 ggagacaaag gcgctatcac tggcccgggc gcttgaggag gccatggagc agaaggcaga
     5281 gctggagcgg ctcaacaagc agttccgcac ggagatggag gacctcatga gctccaagga
     5341 tgacgtgggc aagagtgtcc acgagctgga gaagtccaag cgggccttgg agcagcaggt
     5401 ggaggagatg aagacccagc tggaggagct ggaggatgag ctgcaggcca cggaggatgc
     5461 caagctccgc ctggaggtga acctgcaggc catgaaggcc cagtttgagc gggatctgca
     5521 gggccgggat gaacagagcg aggagaagaa gaagcagctg gtcagacagg tgcgggagat
     5581 ggaggcggag ctggaggatg agaggaagca gcgctccatg gccatggccg cacgcaagaa
     5641 actggagatg gatctgaagg acctggaggc acacattgac acagccaata agaaccggga
     5701 agaggccatc aaacagctgc ggaagcttca ggcccagatg aaggactgca tgcgggagct
     5761 ggacgacacg cgcgcctccc gggaggagat cctggcgcag gccaaggaga atgagaagaa
     5821 gctgaagagc atggaggccg agatgattca gctgcaggag gaactggcag ctgctgagcg
     5881 tgctaagcgt caggcccaac aggaacggga cgagctggct gatgagatcg ccaacagcag
     5941 tggcaaaggg gccctagcat tagaggagaa gcggcgactg gaggcccgca ttgccctgct
     6001 ggaggaggag ctggaggagg aacagggcaa cacggagctg atcaacgatc ggctgaagaa
     6061 ggccaacctg cagatcgacc aaataaacac cgacctgaac ctggaacgca gccacgcaca
     6121 gaagaatgag aatgcgcgac agcagctgga acgccagaac aaggagctca aggccaagct
     6181 gcaggaaatg gagagtgctg tcaagtccaa atacaaggcc tccatcgcgg ccttggaggc
     6241 caaaattgca cagctggagg aacagctgga caacgagacc aaggagcgcc aggcagcctc
     6301 caagcaggtg cgccggacgg agaagaagct gaaggacgtg ctgctgcagg tggaggacga
     6361 gcggaggaac gcggaacagt tcaaggacca ggctgacaag gcgtccaccc gcctgaagca
     6421 gcttaaacgg cagctagagg aggctgaaga ggaggcccag cgggccaatg cctcacgccg
     6481 gaagctgcag cgtgagctgg aagatgccac agagaccgct gatgctatga accgcgaggt
     6541 cagctccctg aagaacaaac tgaggcgtgg ggacctgcca tttgtcgtga ctcgccgaat
     6601 tgttcggaaa ggcactggcg actgctcaga cgaggaggtc gacggtaaag cagatggggc
     6661 cgatgccaag gcagctgaat aggaattctg cagtcgacgg taccgcgggc ccgggatcca
     6721 ccggatctag ataactgatc ataatcagcc ataccac
//
