LOCUS       sequence-85721-e        2328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..744
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtgt ctaagggcga agagctgatt
       61 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
      121 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      181 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
      241 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
      301 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      361 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      421 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
      481 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
      541 ggcgggagcc atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag
      601 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
      661 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
      721 agcaaactgg ggcacaagct taattccgga agtgctggga gcgcaagcgg aagcggaggc
      781 tccggtttta actacgaacg tccaaaacac ttcatccagt cccaaaaccc atgtggctcc
      841 agattgcagc ctcctggacc agaaacctcc agcttctcta gccagaccaa acagtcttcc
      901 attatcatcc agccccgcca gtgtacagag caaagatttt ctgcctcctc aacactgagc
      961 tctcacatca ccatgtcctc ctctgctttc cctgcttctc cccagcagca tgctggctcc
     1021 aacccaggcc aaagggttac aaccacctat aaccagtccc cagccagctt cctcagctcc
     1081 atattaccat cacagcctga ttacaatagc agtaaaatcc cttccgctat ggattccaac
     1141 tatcaacagt cctcagctgg ccaacctata aatgcaaagc catcccaaac tgcaaatgct
     1201 aagcccatac caagaactcc tgatcatgaa atacaaggat caaaagaagc tttgattcaa
     1261 gatttggaaa gaaagctgaa atgcaaggac acccttcttc ataatggaaa tcaacgtcta
     1321 acatatgaag agaagatggc tcgcagattg ctaggaccac agaatgcagc tgctgtgttt
     1381 caagctcagg atgacagtgg tgcacaagac tcgcagcaac acaactcaga acatgcgcga
     1441 ctgcaagttc ctacatcaca agtaagaagt agatcaacct caaggggaga tgtgaatgat
     1501 caggatgcaa tccaggagaa attttaccca ccacgtttca ttcaagtgcc agagaacatg
     1561 tcgattgatg aaggaagatt ctgcagaatg gacttcaaag tgagtggact gccagctcct
     1621 gatgtgtcat ggtatctaaa tggaagaaca gttcaatcag atgatttgca caaaatgata
     1681 gtgtctgaga agggtcttca ttcactcatc tttgaagtag tcagagcttc agatgcaggg
     1741 gcttatgcat gtgttgccaa gaatagagca ggagaagcca ccttcactgt gcagctggat
     1801 gtccttgcaa aagaacataa aagagcacca atgtttatct acaaaccaca gagcaaaaaa
     1861 gttttagagg gagattcagt gaaactagaa tgccagatct cggctatacc tccaccaaag
     1921 cttttctgga aaagaaataa tgaaatggta caattcaaca ctgaccgaat aagcttatat
     1981 caagataaca ctggaagagt tactttactg ataaaagatg taaacaagaa agatgctggg
     2041 tggtatactg tgtcagcagt taatgaagct ggagtgacta catgtaacac aagattagac
     2101 gttacggcac gtccaaacca aactcttcca gctcctaagc agttacgggt tcgaccaaca
     2161 ttcagcaaat atttagcact taatgggaaa ggtttgaatg taaaacaagc ttttaaccca
     2221 gaaggagaat ttcagcgttt ggcagctcaa tctggactct atgaaagtga agaactttaa
     2281 ggatccaccg gatctagata actgatcata atcagccata ccacattt
//
