LOCUS       sequence-85723-e        2829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..742
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgacgctca gatccgctag cggtggccac catggtgtct aagggcgaag agctgattaa
       61 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
      121 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
      181 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
      241 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
      301 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      361 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
      421 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
      481 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
      541 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
      601 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
      661 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
      721 caaactgggg cacaagctta attccggact cagatctcga gtgagcgctc tggcctcccg
      781 cgctaccgcc atgcagtcct ccggctcctt caactacgcg cgccctaagc agttcatcgc
      841 ggcgcagaac ctgggtcccg catcggggct gcccacgcca acctccagcc ccagctcctc
      901 cagcttgccg tcaccattgt cccccacgcc caggcccttc ggccgcgcgc ccgggccgcc
      961 cttcgtggaa cccgaggcca tgtggggccc gtcctcgccc tcgccgccac cgccgccacc
     1021 tccggtcttc agcccctctg cagcctaccc ggtgcccgac gtgttcccac tgccaccgcc
     1081 gccgccaccg ctacctagct ccacctcaca ctgcgcctcg cccgcccgct tcggccccag
     1141 ccagacgccc gcagccttcc tcagcgccct gctgccctct cagccaccgc ctgtcgctgt
     1201 caacgccctg gggctgccca agggagtcac ccccgcgggg ttcccaaaga agtccagtag
     1261 aactgctaga attgcctctg atgaggagat tcaaggcaca aaggatgctg tcatccaaga
     1321 cctggaacgg aagcttcgct tcaaggagga ccttctgaac aatggccaac cgaggctaac
     1381 ctatgaggaa agaatggctc gccgcctgct tggagccgac agcgcaaacg tcttcaacat
     1441 ccaggagcca gaggaaacgg cagccaatca ggagtacaaa gtctctagct gcgagcagag
     1501 gctgattagc gagattgaat acaggctgga gcgctcccct gtggatgagt cgggagacga
     1561 ggtgcaggat ccagatgtgc ctgtggagaa cgcaacagct cccttctttg agatgaagct
     1621 gaaacactac aagatctttg aggggatgcc ggtgactttc acgtgtcgag tggctgggaa
     1681 tccaaagcca aagatctatt ggtttaaaga tgggaagcag atttctccga agagcgatca
     1741 ctacaccatt cagagagacc ttgatgggac ctgctctctc cacaccacgg cctctaccct
     1801 agacgacgat gggaactaca ccatcatggc tgccaaccct cagggtcgcg tcagttgtac
     1861 aggaaggcta atggtacagg ctgtcaacca aagaggccgc agtccccgct ctccctcagg
     1921 ccatcctcat gccagaaggc ctcgctctcg atcacgggac agtggagatg aaaacgagcc
     1981 cattcaggag cgattcttca gacctcactt cctgcaggct cctggagacc tgaccgttca
     2041 ggaaggcaag ctctgcagga tggactgcaa ggtcagtggg ttaccaaccc cagatctcag
     2101 ctggcaacta gacggaaagc ccatacgccc cgacagtgct cacaagatgc tggtccgtga
     2161 gaatggggtc cactccctca ttatagagcc agtcacgtcc cgggacgccg gcatctacac
     2221 atgtattgcc accaacagag caggacagaa ctcgtttaac ctggagcttg tggttgctgc
     2281 taaggaagca cacaaggccc ctgtgtttat ggagaagcta cagaacacgg gggttgctga
     2341 tggataccca gtgcggctag aatgccgtgt ctccggagtg ccgccacctc agatattttg
     2401 gaagaaagaa aatgaatcgc tcactcatag cactgagcga gtaagcatgc accaggataa
     2461 tcatggctac atctgcctgc tcatccaggg agccacaaag gaagacgctg ggtggtacac
     2521 tgtgtctgcc aagaacgaag caggcattgt gtcctgcact gcccggctgg atgtctacac
     2581 ccagtggcac cagcagccac agaccaccaa gccaaaaaaa gtacggccct cggccagtcg
     2641 ctacgcagca ctttcggacc agggactaga catcaaagcc gctttccaac ctgaagccag
     2701 cccatctcac ctgactctga acagcggctt ggtagaaagt gaagacctgt gagaattctg
     2761 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     2821 ataccacat
//
