LOCUS       sequence-85727-e        1455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..739
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgg tggccaccat ggtgtctaag ggcgaagagc tgattaagga
       61 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
      121 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      181 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
      241 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
      301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      361 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
      421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
      481 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
      541 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
      601 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
      661 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
      721 actggggcac aagcttaatt ccggactcag atctcgagcc atggctagtc gaggcgcaac
      781 aagacccaac gggccaaata ctggaaataa aatatgccag ttcaaactag tacttctggg
      841 agagtctgct gttggcaaat caagcctagt gcttcgtttt gtgaagggcc aatttcatga
      901 atttcaagag agtaccatag gggctgcttt tctaacccaa actgtgtgtc ttgatgatac
      961 aacagtaaag tttgaaatat gggatacagc tggtcaagaa cgataccata gcttagcacc
     1021 aatgtactac agaggagcac aagcagccat agttgtatat gatatcacaa atgaggagtc
     1081 ctttgccaga gccaaaaact gggttaaaga acttcagagg caagccagtc ctaacattgt
     1141 aatagcttta tcaggaaaca aggctgatct tgcaaataaa agagctgtcg atttccagga
     1201 agcacagtcc tatgcagatg acaacagttt attattcatg gagacatcag ctaaaacatc
     1261 gatgaatgta aatgaaatat tcatggcaat agctaaaaag ttgccaaaga acgaaccaca
     1321 gaatccagga gcaaattctg ccagaggaag aggagtagac cttactgaac ccacgcagcc
     1381 aaccaggaat cagtgttgta gtaactaagg atccaccgga tctagataac tgatcataat
     1441 cagccatacc acatg
//
