LOCUS       sequence-85731-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1376..1385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1382..2092
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggactc agatctcgag accgccatgg ccactgttac
       61 ctatccttca tctgagccta cgaccttgga ccctgggaac gcatcctcga cctggcccct
      121 ggataccacc ctggggaaca catccgctgg cgctagcctg acaggcctgg ctgtcagtgg
      181 catcttgatc tctctggtgt acctggtggt gtgcgtggtg ggtttgctgg gcaactcgct
      241 ggtgatctac gtggtcctgc ggcacacgtc cagcccatca gtgaccagtg tctatatcct
      301 caacctggct ctggctgatg agctcttcat gctagggcta cccttcctgg ctgctcagaa
      361 cgccctgtcc tactggccct tcggatctct catgtgccgt ctggtcatgg ccgtggatgg
      421 catcaaccag ttcaccagca tcttctgcct caccgtcatg agtgtggacc gctatctggc
      481 tgtggtgcac cccacacgct cagcccgctg gcgcacggca ccagtggctc gcacggtcag
      541 tgcagctgtc tgggtggcct cggctgtggt ggtgctgcct gtggttgtgt tctcaggagt
      601 gccccggggc atgagcacgt gccacatgca gtggccagag ccagcggctg cctggcgaac
      661 agccttcatc atctacacgg ccgcactggg cttctttggg cccctgctgg tcatctgctt
      721 gtgctacttg ctcattgtgg taaaggtgcg gtcgaccacc cggcgggtgc gggcgccctc
      781 gtgtcagtgg gtacaggcac ccgcatgcca gcggcgacgc cgctctgagc gcagggtcac
      841 acgcatggtg gtggccgtgg tggcactctt cgtcctctgc tggatgcctt tctatctgct
      901 caacatcgtc aatgtggtgt gcccgctgcc ggaggagccc gccttcttcg gcctctactt
      961 cctggtggtg gcgctgccct acgccaacag ctgcgcaaac cccatcctct acggcttcct
     1021 ctcctaccgc ttcaagcagg gctttcgcag gatcctgcta agaccatcac gtcgcattcg
     1081 gagccaggag ccagggtcgg gacctccaga gaagactgaa gaggaggagg atgaagaaga
     1141 agaagagaga agggaagagg aggagcggag gatgcagaga gggcaggaga tgaacgggag
     1201 gctcagtcag atcgcacagg ctggcactag tggacaacag ccacggccct gcacagggac
     1261 tgctaaggag cagcagcttc tgccccagga ggccacagct ggggacaagg ccagcacact
     1321 gagccatctg ggaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     1381 catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
     1441 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1501 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     1561 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     1621 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     1681 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1741 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     1801 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     1861 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac
     1921 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     1981 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     2041 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta attaagcggc
     2101 cgcgactcta gatcataatc agccatacca
//
