LOCUS       sequence-85733-e        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 399, 
            Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      483..492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..1199
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc gccggtgtat
       61 gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga agtgacccca
      121 acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag gagagagctg
      181 ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc ttccttgaag
      241 aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc gtagaccatc
      301 tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc ttacagcaaa
      361 ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt agtcagagaa
      421 ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg gatccaccgg
      481 tcgccaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt
      541 acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc
      601 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
      661 ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc
      721 agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca
      781 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
      841 gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc
      901 agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc
      961 tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg
     1021 ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact
     1081 atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc
     1141 acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt
     1201 aagcggccgc gactctagat cataatcagc cataccacat t
//
