LOCUS       sequence-85738-e        2232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 399, Emission: 
            456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1472..1481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1478..2188
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcccat
       61 gtccaccagg tccgtgtcct cgtcctccta ccgcaggatg ttcggcggcc cgggcaccgc
      121 gagccggccg agctccagcc ggagctacgt gactacgtcc acccgcacct acagcctggg
      181 cagcgcgctg cgccccagca ccagccgcag cctctacgcc tcgtccccgg gcggcgtgta
      241 tgccacgcgc tcctctgccg tgcgcctgcg gagcagcgtg cccggggtgc ggctcctgca
      301 ggactcggtg gacttctcgc tggccgacgc catcaacacc gagttcaaga acacccgcac
      361 caacgagaag gtggagctgc aggagctgaa tgaccgcttc gccaactaca tcgacaaggt
      421 gcgcttcctg gagcagcaga ataagatcct gctggccgag ctcgagcagc tcaagggcca
      481 aggcaagtcg cgcctggggg acctctacga ggaggagatg cgggagctgc gccggcaggt
      541 ggaccagcta accaacgaca aagcccgcgt cgaggtggag cgcgacaacc tggccgagga
      601 catcatgcgc ctccgggaga aattgcagga ggagatgctt cagagagagg aagccgaaaa
      661 caccctgcaa tctttcagac aggatgttga caatgcgtct ctggcacgtc ttgaccttga
      721 acgcaaagtg gaatctttgc aagaagagat tgcctttttg aagaaactcc acgaagagga
      781 aatccaggag ctgcaggctc agattcagga acagcatgtc caaatcgatg tggatgtttc
      841 caagcctgac ctcacggctg ccctgcgtga cgtacgtcag caatatgaaa gtgtggctgc
      901 caagaacctg caggaggcag aagaatggta caaatccaag tttgctgacc tctctgaggc
      961 tgccaaccgg aacaatgacg ccctgcgcca ggcaaagcag gagtccactg agtaccggag
     1021 acaggtgcag tccctcacct gtgaagtgga tgcccttaaa ggaaccaatg agtccctgga
     1081 acgccagatg cgtgaaatgg aagagaactt tgccgttgaa gctgctaact accaagacac
     1141 tattggccgc ctgcaggatg agattcagaa tatgaaggag gaaatggctc gtcaccttcg
     1201 tgaataccaa gacctgctca atgttaagat ggcccttgac attgagattg ccacctacag
     1261 gaagctgctg gaaggcgagg agagcaggat ttctctgcct cttccaaact tttcctccct
     1321 gaacctgagg gaaactaatc tggattcact ccctctggtt gatacccact caaaaaggac
     1381 acttctgatt aagacggttg aaactagaga tggacaggtt atcaacgaaa cttctcagca
     1441 tcacgatgac cttgaagggg atccaccggt cgccaccatg gtgtctaagg gcgaagagct
     1501 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     1561 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1621 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     1681 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     1741 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1801 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1861 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     1921 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     1981 cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc
     2041 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     2101 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     2161 ccctagcaaa ctggggcaca agcttaatta agcggccgcg actctagatc ataatcagcc
     2221 ataccacatt ga
//
