LOCUS       sequence-85739-e        4041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..732
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gatccgctag cggtggccac catggtgtct aagggcgaag agctgattaa ggagaacatg
       61 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag
      121 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
      181 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc
      241 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca
      301 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      361 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac
      421 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc
      481 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat
      541 ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg
      601 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc
      661 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg
      721 cacaagctta attccggact cagatctcga gctcaagctt cgaattcggg aaactcgaga
      781 acaaagcttg aaaacggccg catgccagtg tttcatacgc gcacgatcga gagcatcctg
      841 gagccggtgg cacagcagat ctcccacctg gtgataatgc acgaggaggg cgaggtggac
      901 ggcaaagcca ttcctgacct caccgcgccc gtggccgccg tgcaggcggc cgtcagcaac
      961 ctcgtccggg ttggaaaaga gactgttcaa accactgagg atcagatttt gaagagagat
     1021 atgccaccag catttattaa ggttgagaat gcttgcacca agcttgtcca ggcagctcag
     1081 atgcttcagt cagaccctta ctcagtgcct gctcgagatt atctaattga tgggtcaagg
     1141 ggcatcctct ctggaacatc agacctgctc cttaccttcg atgaggctga ggtccgtaaa
     1201 attattagag tttgcaaagg aattttggaa tatcttacag tggcagaggt ggtggagact
     1261 atggaagatt tggtcactta cacaaagaat cttgggccag gaatgactaa gatggccaag
     1321 atgattgacg agagacagca ggagctcact caccaggagc accgagtgat gttggtgaac
     1381 tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt cagctatgaa gatttttgta
     1441 acaactaaaa actcaaaaaa ccaaggcata gaggaagctt taaaaaatcg caattttact
     1501 gtagaaaaaa tgagtgctga aattaatgag ataattcgtg tgttacaact cacctcttgg
     1561 gatgaagatg cctgggccag caaggacact gaagccatga agagagcatt ggcctccata
     1621 gactccaaac tgaaccaggc caaaggttgg ctccgtgacc ctagtgcctc cccaggggat
     1681 gctggtgagc aggccatcag acagatctta gatgaagctg gaaaagttgg tgaactctgt
     1741 gcaggcaaag aacgcaggga gattctggga acttgcaaaa tgctagggca gatgactgat
     1801 caagtggctg acctccgtgc cagaggacaa ggatcctcac cggtggccat gcagaaagct
     1861 cagcaggtat ctcagggtct ggatgtgctc acagcaaaag tggaaaatgc agctcgcaag
     1921 ctggaagcca tgaccaactc aaagcagagc attgcaaaga agatcgatgc tgctcagaac
     1981 tggcttgcag atccaaatgg tggaccggaa ggagaagagc agattcgagg tgctttggct
     2041 gaagctcgga aaatagcaga attatgtgat gatcctaaag aaagagatga cattctacgt
     2101 tcccttgggg aaatatctgc tctgacttct aaattagcag atctacgaag acaggggaaa
     2161 ggagattctc cagaggctcg agccttggcc aaacaggtgg ccacggccct gcagaacctg
     2221 cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg ccaaagcagc tgtacacctt
     2281 gagggcaaga ttgagcaagc acagcggtgg attgataatc ccacagtgga tgaccgtgga
     2341 gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc atcgtctggc taatgttatg
     2401 atggggcctt atcggcaaga tcttctcgcc aagtgtgacc gagtggacca gctgacagcc
     2461 cagctggctg acctggctgc cagaggggaa ggggagagtc ctcaggcacg agcacttgca
     2521 tctcagctcc aagactcctt aaaggatcta aaagctcgga tgcaggaggc catgactcag
     2581 gaagtgtcag atgttttcag cgataccaca actcccatca agctgttggc agtggcagcc
     2641 acggcgcctc ctgatgcgcc taacagggaa gaggtatttg atgagagggc agctaacttt
     2701 gaaaaccatt caggaaagct tggtgctacg gccgagaagg cggctgcggt tggtactgct
     2761 aataaatcaa cagtggaagg cattcaggcc tcagtgaaga cggcccgaga actcacaccc
     2821 caggtggtct cggctgctcg tatcttactt aggaaccctg gaaatcaagc tgcttatgaa
     2881 cattttgaga ccatgaagaa ccagtggatc gataatgttg aaaaaatgac agggctggtg
     2941 gacgaagcca ttgataccaa atctctgttg gatgcttcag aagaagcaat taaaaaagac
     3001 ctggacaagt gcaaggtagc tatggccaac attcagcctc agatgctggt tgctggggca
     3061 accagtattg ctcgtcgggc caaccggatc ctgctggtgg ctaagaggga ggtggagaat
     3121 tccgaggatc ccaagttccg tgaggctgtg aaagctgcct ctgatgaatt gagcaaaacc
     3181 atctccccga tggtgatgga tgcaaaagct gtggctggaa acatttccga ccctggactg
     3241 caaaagagct tcctggactc aggatatcgg atcctgggag ctgtggccaa ggtcagagaa
     3301 gccttccaac ctcaggagcc tgacttcccg ccgcctccac cagaccttga acaactccga
     3361 ctaacagatg agcttgctcc tcccaaacca cctctgcctg aaggtgaggt ccctccacct
     3421 aggcctccac caccagagga aaaggatgaa gagttccctg agcagaaggc cggggaggtg
     3481 attaaccagc caatgatgat ggctgccaga cagctccatg atgaagctcg caaatggtcc
     3541 agcaagggca atgacatcat tgcagcagcc aagcgcatgg ctctgctgat ggctgagatg
     3601 tctcggctgg taagaggggg cagtggtacc aagcgggcac tcattcagtg tgccaaggac
     3661 atcgccaagg cctcagatga ggtgactcgg ttggccaagg aggttgccaa gcagtgcaca
     3721 gataaacgga ttagaaccaa cctcttacag gtatgtgagc gaatcccaac cataagcacc
     3781 cagctcaaaa tcctgtccac agtgaaggcc accatgctgg gccggaccaa catcagtgat
     3841 gaggagtctg agcaggccac agagatgctg gttcacaatg cccagaacct catgcagtct
     3901 gtgaaggaga ctgtgcggga agctgaagct gcttcaatca aaattcgaac agatgctgga
     3961 tttacactgc gctgggttag aaagactccc tggtaccagt aggcggccgc gactctagat
     4021 cataatcagc cataccacat t
//
