LOCUS       sequence-85740-e        6042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..745
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     RBS             2446..2454
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg tctaagggcg aagagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      241 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      481 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      541 gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa
      601 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      661 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaagc ttaattccgg aagtgctggg agcgcaagcg gaagcggagg
      781 ctccggtgag gaaacagcta tatgggaaca acatacagtg acgcttcaca gggctcctgg
      841 atttggattt ggaattgcaa tatctggtgg acgagataat cctcattttc agagtgggga
      901 aacgtcaata gtgatttcag atgtgctgaa aggaggacca gctgaaggac agctacagga
      961 aaatgaccga gttgcaatgg ttaacggagt ttcaatggat aatgttgaac atgcttttgc
     1021 tgttcagcaa ctaaggaaaa gtgggaaaaa tgcaaaaatt acaattagaa ggaagaagaa
     1081 agttcaaata ccagtaagtc gtcctgatcc tgaaccagta tctgataatg aagaagatag
     1141 ttatgatgag gaaatacatg atccaagaag tggccggagt ggtgtggtta acagaaggag
     1201 tgagaagatt tggccgaggg atagaagtgc aagtagagag aggagcttgt ccccgcggtc
     1261 agacaggcgg tcagtggctt ccagccagcc tgctaaacct actaaagtca cactggtgaa
     1321 atcccggaaa aatgaagaat atggtcttcg attggcaagc catatatttg ttaaggaaat
     1381 ttcacaagat agtttggcag caagagatgg caatattcaa gaaggtgatg ttgtattgaa
     1441 gataaatggt actgtgacag aaaatatgtc attgacagat gcaaagacat tgatagaaag
     1501 gtctaaaggc aaattaaaaa tggtagttca aagagatgaa cgggctacgc tattgaatgt
     1561 ccctgatctt tctgacagca tccactctgc taatgcctct gagagagacg acatttcaga
     1621 aattcagtca ctggcatcag atcattctgg tcgatcacac gataggcctc cccgccgcag
     1681 ccggtcacga tctcctgacc agcggtcaga gccttctgat cattccaggc actcgccgca
     1741 gcagccaagc aatggcagtc tgcggagtag agatgaagag agaatttcta aacctggggc
     1801 tgtctcaact cctgtaaagc atgctgatga tcacacacct aaaacagtgg aagaagttac
     1861 agttgaaaga aatgagaaac aaacaccttc tcttccagaa ccaaagcctg tgtatgccca
     1921 agttggcaac cagatgtgga tttacctgtc agtccatctg atggtgtcct acctaattca
     1981 actcatgaag atgggatttc ttcggcccag catgaaattg gtaaaattca gaaaaggaga
     2041 tagtgtgggt ttgcggctgg ctggtggaaa tgatgttgga atatttgtag ctggcgttct
     2101 agaagatagc cctgcagcca aggaaggctt agaggaaggt gatcaaattc tcagggtaaa
     2161 caacgtagat tttacaaata tcataagaga agaagccgtc cttttcctgc ttgacctccc
     2221 taaaggagaa gaagtgacca tattggctca gaagaagaag gatgtttatc gtcgcattgt
     2281 agaatcagat gtaggagatt ctttctatat tagaacccat tttgaatatg aaaaggaatc
     2341 tccctatgga cttagtttta acaaaggaga ggtgttccgt gctgtggata ccttgtacaa
     2401 tggaaaactg ggctcttggc ttgctattcg aattggtaaa aatcataagg aggtagaacg
     2461 aggcatcatc cctaataaga acagagctga gcagctcgcc agtgtacagt atacacttcc
     2521 aaaaacagca ggcggagacc gtgctgactt ctggagattc agaggtcttc gcagctccaa
     2581 gagaaatctt cgaaaaagca gagaggattt gtccgctcag cctgttcaaa caaagtttcc
     2641 agcttatgaa agagtggttc ttcgagaagc tggatttctg aggcctgtaa ccatttttgg
     2701 accaatagct gatgttgcca gagaaaagct ggcaagagaa gaaccagata tttatcaaat
     2761 tgcaaagagt gaaccacgag acgctggaac tgaccaacgt agctctggct atattcgcct
     2821 gcatacaata aagcaaatca tagatcaaga caaacatgct ttattagatg taacaccaaa
     2881 tgcagttgat cgtcttaact atgcccagtg gtatccaatt gttgtatttc ttaaccctga
     2941 ttctaagcaa ggagtaaaaa caatgagaat gaggttatgt ccagaatctc ggaaaagtgc
     3001 caggaagtta tacgagcgat ctcataaact tgctaaaaat aatcaccatc tttttacaac
     3061 tacaattaac ttaaattcaa tgaatgatgg ttggtatggt gcgctgaaag aagcagttca
     3121 acaacagcaa aaccagctgg tatgggtttc cgagggaaag gcggatggtg ctacaagtga
     3181 tgaccttgat ttgcatgatg atcgtctgtc ctacctgtca gctccaggta gtgaatactc
     3241 aatgtatagc acggacagta gacacacttc tgactatgaa gacacagaca cagaaggcgg
     3301 ggcctacact gatcaagaac tagatgaaac tcttaatgat gaggttggga ctccaccgga
     3361 gtctgccatt acacggtcct ctgagcctgt aagagaggac tcctctggaa tgcatcatga
     3421 aaaccaaaca tatcctcctt actcaccaca agcgcagcca caaccaattc atagaataga
     3481 ctcccctgga tttaagccag cctctcaaca gaaagcagaa gcttcatctc cagtccctta
     3541 cctttcgcct gaaacaaacc cagcatcatc aacctctgct gttaatcata atgtaaattt
     3601 aactaatgtc agactggagg agcccacccc agctccttcc acctcttact caccacaagc
     3661 tgattcttta agaacaccaa gtactgaggc agctcacata atgctaagag atcaagaacc
     3721 atcattgtcg tcgcatgtag atccaacaaa ggtgtataga aaggacccat atcccgagga
     3781 aatgatgagg cagaaccatg ttttgaaaca gccagccgtt agtcacccag ggcacaggcc
     3841 agacaaagag cctaatctga cctatgaacc ccaactccca tacgtagaga aacaagccag
     3901 cagagacctg gagcagccca catacagata cgagtcctca agctatacgg accagttttc
     3961 tcgaaactat gaacatcgtc tgcgatacga agatcgcgtc cccatgtatg aagaacagtg
     4021 gtcatattat gatgacaaac agccctaccc atctcggcca ccttttgata atcagcactc
     4081 tcaagacctt gactccagac agcatcccga agagtcctca gaacgagggt actttccacg
     4141 ttttgaagag ccagcccctc tgtcttacga cagcagacca cgttacgaac aggcacctag
     4201 agcatccgcc ctgcggcacg aagagcagcc agctcctggg tatgacacac atggtagact
     4261 cagaccggaa gcccagcccc acccttcagc agggcccaag cctgcagagt ccaagcagta
     4321 ttttgagcaa tattcacgca gttacgagca agtaccaccc caaggattta cctctagagc
     4381 aggtcatttt gagcctctcc atggtgctgc agctgtccct ccgctgatac cttcatctca
     4441 gcataagcca gaagctctgc cttcaaacac caaaccactg cctccacccc caactcaaac
     4501 cgaagaagag gaagatccag caatgaagcc acagtctgta ctcaccagag ttaagatgtt
     4561 tgaaaacaaa aggtctgcat ccttagagac caagaaggat gtaaatgaca ctggcagttt
     4621 taagcctcca gaagtagcat ctaaaccttc aggtgctccc atcattggtc ccaaacccac
     4681 ttctcagaat caattcagtg aacatgacaa aactctgtac aggattccag aacctcaaaa
     4741 acctcaactg aagccacctg aagatattgt tcggtccaat cattatgacc ctgaagaaga
     4801 tgaagaatat tatcgaaaac agctgtcata ctttgaccga agaagttttg agaataagcc
     4861 tcctgcacac attgctgcca gccatctctc cgagcctgca aagccagctc attctcagaa
     4921 tcaatcaaat ttttctagtt attcttcaaa gggaaagcct cctgaagctg atggtgtgga
     4981 tagatcattt ggcgagaaac gctatgaacc catccaggcc actccccctc ctcctccatt
     5041 gccctcgcag tatgcccagc catctcagcc tgtcaccagc gcgtctctcc acatacattc
     5101 taagggagca catggtgaag gtaattcagt gtcattggat tttcagaatt ccttagtgtc
     5161 caaaccagac ccacctccat ctcagaataa gccagcaact ttcagaccac caaaccgaga
     5221 agatactgct caggcagctt tctatcccca gaaaagtttt ccagataaag ccccagttaa
     5281 tggaactgaa cagactcaga aaacagtcac tccagcatac aatcgattca caccaaaacc
     5341 atatacaagt tctgcccgac catttgaacg caagtttgaa agtcctaaat tcaatcacaa
     5401 tcttctgcca agtgaaactg cacataaacc tgacttgtct tcaaaaactc ccacttctcc
     5461 aaaaactctt gtgaaatcgc acagtttggc acagcctcct gagtttgaca gtggagttga
     5521 aactttctct atccatgcag agaagcctaa atatcaaata aataatatca gcacagtgcc
     5581 taaagctatt cctgtgagtc cttcagctgt ggaagaggat gaagatgaag atggtcatac
     5641 tgtggtggcc acagcccgag gcatatttaa cagcaatggg ggcgtgctga gttccataga
     5701 aactggtgtt agtataatta tccctcaagg agccattccc gaaggagttg agcaggaaat
     5761 ctatttcaag gtctgccggg acaacagcat ccttccacct ttagataaag agaaaggtga
     5821 aacactgctg agtcctttgg tgatgtgtgg tccccatggc ctcaagttcc tgaagcctgt
     5881 ggagctgcgc ttaccacact gtgatcctaa aacctggcaa aacaagtgtc ttcccggaga
     5941 tccaaattat ctcgttggag caaactgtgt ttctgtcctt attgaccact tttaaggatc
     6001 caccggatct agataactga tcataatcag ccataccaca tt
//
