LOCUS       sequence-85743-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MiCy-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..927
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctgg tacccttgca
       61 gcttccagtt gtcaggaaga gaaggctccc atggatcctg tggtggtgct ggggctctgt
      121 ctctcctgtt tgcttctcct ttcactctgg aaacagagct atgggggagg gaagcttcga
      181 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtcttat
      241 tcaaagcaag gcatcgcaca agaaatgcgg acgaaatacc gtatggaagg cagtgtcaat
      301 ggccatgagt tcacgatcga aggtgtagga actggaaacc cttacgaagg gaaacagatg
      361 tccgaattag tgatcatcaa gcctaaggga aaaccccttc cattctcctt tgacatactg
      421 tcaacagcct ttcaatatgg aaacagatgc ttcacaaagt accctgccga catgcctgac
      481 tatttcaagc aagcattccc agatggaatg tcatatgaaa ggtcatttct atttgaggat
      541 ggaggagttg ctacagccag ctggagcatt cgtctcgaag gaaattgctt catccacaat
      601 tccatctatc atggcgtaaa ctttcccgct gatggacccg taatgaagaa gcagacaatt
      661 ggctgggata agtccttcga aaaaatgagt gtggctaaag aggtgctaag aggtgatgtg
      721 actcagtttc ttctgctcga aggaggtggt taccagagat gccggtttca ctccacttac
      781 aaaacggaga agccagtcgc aatgcccccg agtcatgtcg tagaacatca aattgtgagg
      841 accgaccttg gccaaactgc aaaaggcttc aaggtcaagc tggaagaaca tgctgaggct
      901 catgttaacc ctttgaaggt taaataagcg gccgcgactc tagatcataa tcagccatac
      961 cacatgagag ttgcc
//
