LOCUS       sequence-85744-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MiCy-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..453
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      466..475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             472..1170
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc
       61 gacggtaccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc
      121 aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag
      181 gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt
      241 tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca
      301 ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc
      361 cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt
      421 actaaggcca tcaccaagta caccagcgct aaggatccac cggtcgccac catggtgtct
      481 tattcaaagc aaggcatcgc acaagaaatg cggacgaaat accgtatgga aggcagtgtc
      541 aatggccatg agttcacgat cgaaggtgta ggaactggaa acccttacga agggaaacag
      601 atgtccgaat tagtgatcat caagcctaag ggaaaacccc ttccattctc ctttgacata
      661 ctgtcaacag cctttcaata tggaaacaga tgcttcacaa agtaccctgc cgacatgcct
      721 gactatttca agcaagcatt cccagatgga atgtcatatg aaaggtcatt tctatttgag
      781 gatggaggag ttgctacagc cagctggagc attcgtctcg aaggaaattg cttcatccac
      841 aattccatct atcatggcgt aaactttccc gctgatggac ccgtaatgaa gaagcagaca
      901 attggctggg ataagtcctt cgaaaaaatg agtgtggcta aagaggtgct aagaggtgat
      961 gtgactcagt ttcttctgct cgaaggaggt ggttaccaga gatgccggtt tcactccact
     1021 tacaaaacgg agaagccagt cgcaatgccc ccgagtcatg tcgtagaaca tcaaattgtg
     1081 aggaccgacc ttggccaaac tgcaaaaggc ttcaaggtca agctggaaga acatgctgag
     1141 gctcatgtta accctttgaa ggttaaataa gcggccgcga ctctagatca taatcagcca
     1201 taccaca
//
