LOCUS       sequence-85746-e        1502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1502)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ECFP-ELP1-25
FEATURES             Location/Qualifiers
     source          1..1502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      737..746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(743..745,746..748,749..1462)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(788..809)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1049..1068)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1396..1417
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgccg ccaccatggc catgaacatt ttccggctga ctggggacct
       61 gtcccacctg gcggccatcg tcatcctgct gctgaagatc tggaagacgc gctcctgcgc
      121 cggtatttct gggaaaagcc agcttctgtt tgcactggtc ttcacaactc gttacctgga
      181 tctttttact tcatttattt cattgtataa cacatctatg aaggttatct accttgcctg
      241 ctcctatgcc acagtgtacc tgatctacct gaaatttaag gcaacctacg atggaaatca
      301 tgataccttc cgagtggagt ttctggtggt ccctgtggga ggcctctcat ttttagttaa
      361 tcacgatttc tctcctcttg agatcctctg gaccttctcc atctacctgg agtccgtggc
      421 tatccttccg cagctgttta tgatcagcaa gactggggag gccgagacca tcaccaccca
      481 ctacctgttc ttcctgggcc tctatcgtgc tttgtatctt gtcaactgga tctggcgctt
      541 ctactttgag ggcttctttg acctcattgc tgtggtggcc ggcgtagtcc agaccatcct
      601 atactgtgac ttcttctact tgtacattac aaaagtactc aagggaaaga agctcagttt
      661 gccagcaggc ggtggggggg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      721 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      781 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga
      841 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      901 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
      961 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1021 cgtccaggag cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1081 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1141 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
     1201 caccgccgac aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga
     1261 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1321 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1381 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1441 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1501 tt
//
