LOCUS       sequence-85747-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: ECFP-ER-0
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(88..90,91..801)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(130..151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(391..410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     738..759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    817..873
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcatgc tgctatccgt gccgttgctg
       61 ctcggcctcc tcggcctggc cgtcgccgtg agcaagggcg aggagctgtt caccggggtg
      121 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      181 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      241 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      301 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      361 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      421 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      481 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
      541 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      601 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      661 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      721 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      781 ggcatggacg agctgtacaa gaaggacgag ctgtaaagat ctcgagctca agcttcgaat
      841 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
      901 agccatacca
//
