LOCUS       sequence-85748-e        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ECFP-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(374..376,377..379,380..1093)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(419..440)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(680..699)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1027..1048
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga ggcttcggga
      121 gccgctcctg agcggcagcg ccgcgatgcc aggcgcgtcc ctacagcggg cctgccgcct
      181 gctcgtggcc gtctgcgctc tgcaccttgg cgtcaccctc gtttactacc tggctggccg
      241 cgacctgagc cgcctgcccc aactggtcgg agtctccaca ccgctgcagg gcggctcgaa
      301 cagtgccgcc gccatcgggc agtcctccgg ggagctccgg accggagggg ccaaggatcc
      361 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      421 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      481 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      541 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      601 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      661 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      721 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      781 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      841 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
      901 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      961 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1021 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1081 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     1141 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
//
