LOCUS       sequence-85750-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ECFP-Mito-7
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..72
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     23..43
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             101..175
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      203..212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(209..211,212..214,215..928)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(254..275)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(515..534)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     862..883
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
       61 ataagcagag ctggtttagt gaaccgtcag atccgctagc atgtccgtcc tgacgccgct
      121 gctgctgcgg ggcttgacag gctcggcccg gcggctccca gtgccgcgcg ccaagatcca
      181 ttcgttgggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
      421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
      661 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
      721 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      781 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      901 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
      961 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1021 aaacataaaa tgaatgcaat tgtt
//
