LOCUS       sequence-85753-e        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cerulean-H2B-6
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..450
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      463..472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(469..471,472..474,475..1188)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(514..535)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(775..794)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1122..1143
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
       61 ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag
      121 aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag
      181 agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg
      241 tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt
      301 gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag
      361 acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact
      421 aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      901 aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      961 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1021 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1081 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1141 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     1201 tctagatcat aatcagccat accacattt
//
