LOCUS       sequence-85756-e        3195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3195)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(330..349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      795..804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             846..881
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
       61 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      181 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg
      241 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      301 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      361 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      421 aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata
      481 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
      541 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      601 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
      661 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
      781 tcgacggtac cgcggccacc atggctactc aagctgacct gatggagttg gacatggcca
      841 tggagccgga cagaaaagct gctgtcagcc actggcagca gcagtcttac ttggattctg
      901 gaatccattc tggtgccacc accacagctc cttccctgag tggcaagggc aaccctgagg
      961 aagaagatgt tgacacctcc caagtccttt atgaatggga gcaaggcttt tcccagtcct
     1021 tcacgcaaga gcaagtagct gatattgacg ggcagtatgc aatgactagg gctcagaggg
     1081 tccgagctgc catgttccct gagacgctag atgagggcat gcagatccca tccacgcagt
     1141 ttgacgctgc tcatcccact aatgtccagc gcttggctga accatcacag atgttgaaac
     1201 atgcagttgt caatttgatt aactatcagg atgacgcgga acttgccaca cgtgcaattc
     1261 ctgagctgac aaaactgcta aacgatgagg accaggtggt agttaataaa gctgctgtta
     1321 tggtccatca gctttccaaa aaggaagctt ccagacatgc catcatgcgc tcccctcaga
     1381 tggtgtctgc cattgtacgc accatgcaga atacaaatga tgtagagaca gctcgttgta
     1441 ctgctgggac tctgcacaac ctttctcacc accgcgaggg cttgctggcc atctttaagt
     1501 ctggtggcat cccagcgctg gtgaaaatgc ttgggtcacc agtggattct gtactgttct
     1561 acgccatcac gacactgcat aatctcctgc tccatcagga aggagctaaa atggcagtgc
     1621 gcctagctgg tggactgcag aaaatggttg ctttgctcaa caaaacaaac gtgaaattct
     1681 tggctattac aacagactgc cttcagatct tagcttatgg caatcaagag agcaagctca
     1741 tcattctggc cagtggtgga ccccaagcct tagtaaacat aatgaggacc tacacttatg
     1801 agaagcttct gtggaccaca agcagagtgc tgaaagtgct gtctgtctgc tctagcaaca
     1861 agccggccat tgtagaagct ggtgggatgc aggcactggg gcttcatctg acagacccaa
     1921 gtcagcgact tgttcaaaac tgtctttgga ctctcagaaa cctttcagat gcagcgacta
     1981 agcaggaagg gatggaaggc ctccttggga ctctagtgca gcttctgggt tccgatgata
     2041 taaatgtggt cacctgtgca gctggaattc tctctaacct cacttgcaat aattacaaaa
     2101 acaagatgat ggtgtgccaa gtgggtggca tagaggctct tgtacgcacc gtccttcgtg
     2161 ctggtgacag ggaagacatc actgagcctg ccatctgtgc tcttcgtcat ctgaccagcc
     2221 ggcatcagga agccgagatg gcccagaatg ccgttcgcct tcattatgga ctgcctgttg
     2281 tggttaaact cctgcaccca ccatcccact ggcctctgat aaaggcaact gttggattga
     2341 ttcgaaacct tgccctttgc ccagcaaatc atgcgccttt gcgggaacag ggtgctattc
     2401 cacgactagt tcagctgctt gtacgagcac atcaggacac ccaacggcgc acctccatgg
     2461 gtggaacgca gcagcagttt gtggagggcg tgcgcatgga ggagatagta gaagggtgta
     2521 ctggagctct ccacatcctt gctcgggacg ttcacaaccg gattgtaatc cgaggactca
     2581 ataccattcc attgtttgtg cagttgcttt attctcccat tgaaaatatc caaagagtag
     2641 ctgcaggggt cctctgtgaa cttgctcagg acaaggaggc tgcagaggcc attgaagctg
     2701 agggagccac agctcccctg acagagttac tccactccag gaatgaaggc gtggcaacat
     2761 acgcagctgc tgtcctattc cgaatgtctg aggacaagcc acaggattac aagaagcggc
     2821 tttcagtcga gctgaccagt tccctcttca ggacagagcc aatggcttgg aatgagactg
     2881 cagatcttgg actggacatt ggtgcccagg gagaagccct tggatatcgc caggatgatc
     2941 ccagctaccg ttcttttcac tctggtggat acggccagga tgccttgggg atggacccta
     3001 tgatggagca tgagatgggt ggccaccacc ctggtgctga ctatccagtt gatgggctgc
     3061 ctgatctggg acacgcccag gacctcatgg atgggctgcc cccaggtgat agcaatcagc
     3121 tggcctggtt tgatactgac ctgtaaaagc ttggatccac cggatctaga taactgatca
     3181 taatcagcca tacca
//
