LOCUS       sequence-85758-e        1838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1838)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1073..1082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1079..1081,1082..1084,1085..1798)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1124..1145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1385..1404)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1732..1753
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccag
       61 gatgtggtgg tccttgatcc ctctctcttg cctgctggca ctgaccagtg cccatgacaa
      121 gccttccttt cacccactgt cggacgacat gattaactat atcaacaaac agaatacaac
      181 atggcaggct ggacgcaact tctacaatgt tgacataagc tatctgaaga agctgtgtgg
      241 aactgtcctg ggtggaccca agctgccgga aagggttggg ttcagcgagg acataaatct
      301 acctgaatcc tttgatgcac gggaacagtg gtccaattgc ccgaccatcg cacagatcag
      361 agaccagggg tcctgtggct cttgttgggc atttggggca gtggaagcca tgtctgaccg
      421 aatctgcatt cacaccaatg gccgagtcaa tgtggaggtg tctgctgagg acctgcttac
      481 ctgctgtggt atccagtgtg gggatggctg taatggtggc tatccctctg gagcatggaa
      541 cttctggact agaaaaggcc tggtttctgg tggagtatac aattctcata taggctgctt
      601 accctacacc atccctccct gtgaacacca tgtcaatggc tcccgtcccc catgcactgg
      661 agaaggagat actcccaagt gcaacaagat gtgtgaggct ggctactcca catcctacaa
      721 ggaagataag cactatgggt acacttccta cagtgtgtct gacagcgaga aggagatcat
      781 ggcggaaatc tacaaaaatg gcccagtgga gggtgctttt actgtgtttt ctgacttctt
      841 gacttacaaa tcaggcgtat acaagcatga agccggtgat gtgatgggag gccatgccat
      901 ccgcattctg ggctggggaa tagagaatgg agtaccctac tggctggtag caaactcctg
      961 gaacgttgac tggggtgata atggtttctt taaaatcctc agaggagaga accactgtgg
     1021 aattgaatca gaaatcgtgg ctggaatccc acgcactcag gatccaccgg tcgccaccat
     1081 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1141 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1201 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1261 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1321 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1381 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1441 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1501 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
     1561 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1621 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1681 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1741 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     1801 cggccgcgac tctagatcat aatcagccat accacatt
//
