LOCUS       sequence-85759-e        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-CD151-7
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac
      481 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagtcc ggactcagat ctggcggtat gggtgagttc
      781 aacgagaaga agacaacatg tggcaccgtt tgcctcaagt acctgctgtt tacctacaat
      841 tgctgcttct ggctggctgg cctggctgtc atggcagtgg gcatctggac gctggccctc
      901 aagagtgact acatcagcct gctggcctca ggcacctacc tggccacagc ctacatcctg
      961 gtggtggcgg gcactgtcgt catggtgact ggggtcttgg gctgctgcgc caccttcaag
     1021 gagcgtcgga acctgctgcg cctgtacttc atcctgctcc tcatcatctt tctgctggag
     1081 atcatcgctg gtatcctcgc ctacgcctac taccagcagc tgaacacgga gctcaaggag
     1141 aacctgaagg acaccatgac caagcgctac caccagccgg gccatgaggc tgtgaccagc
     1201 gctgtggacc agctgcagca ggagttccac tgctgtggca gcaacaactc acaggactgg
     1261 cgagacagtg agtggatccg ctcacaggag gccggtggcc gtgtggtccc agacagctgc
     1321 tgcaagacgg tggtggctct ttgtggacag cgagaccatg cctccaacat ctacaaggtg
     1381 gagggcggct gcatcaccaa gttggagacc ttcatccagg agcacctgag ggtcattggg
     1441 gctgtgggga tcggcattgc ctgtgtgcag gtctttggca tgatcttcac gtgctgcctg
     1501 tacaggagtc tcaagctgga gcactactga gaattctgca gtcgacggta ccgcgggccc
     1561 gggatccacc ggatctagat aactgatcat aatcagcc
//
