LOCUS       sequence-85762-e        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
      781 aattctgcag tcgacatggc tgatgacttt ggcttcttct cgtcgtcgga gagtggtgcc
      841 ccggaggcgg cggaggagga cccggcggcc gccttcctgg cccagcagga gagcgagatt
      901 gcaggcatag agaacgacga gggcttcggg gcacctgccg gcagccatgc ggcccccgca
      961 cagccgggcc ccacgagtgg ggctggttct gaggacatgg ggaccacagt caatggagat
     1021 gtgtttcagg aggccaacgg tcctgctgat ggctacgcag ccattgccca ggctgacagg
     1081 ctgacccagg agcctgagag catccgcaag tggcgagagg agcagaggaa acggctgcaa
     1141 gagctggatg ctgcatctaa ggtcacggaa caggaatggc gggagaaggc caagaaggac
     1201 ctggaggagt ggaaccagcg ccagagtgaa caagtagaga agaacaagat caacaaccgg
     1261 gcatccgagg aggctttcgt gaaggaatcc aaggaggaga ccccaggcac agagtgggag
     1321 aaggtggccc agctatgtga cttcaacccc aagagcagca agcagtgcaa agatgtgtcc
     1381 cgcctgcgct cggtgctcat gtccctgaag cagacgccac tgtcccgcta gggatccacc
     1441 ggatctagat aactgatcat aatcagccat accacatt
//
