LOCUS       sequence-85767-e        2168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2168)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1403..1412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1409..1411,1412..1414,1415..2128)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1454..1475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1715..1734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2062..2083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tcggcagcac
       61 ttttctttca ttggggggaa ggcgtgagga aagtaccaaa cagcagcaga cttttaaact
      121 ttaaacagac aggtctgaga gcctgaactc tcatttttcc tttgacttca gcctccaagg
      181 agttccacca actttggcgc gccggcttca ctttcattaa gtgaaagaga ggtgcccaga
      241 catgggtgac tggagtgcct tggggaagct tctggacaag gtccaagcct actccaccgc
      301 tggagggaag gtgtggctgt cagtgctctt catattcaga atcctgctcc tggggacagc
      361 tgttgagtca gcttggggtg atgaacagtc tgcctttcgc tgtaacactc aacaacctgg
      421 ctgcgaaaac gtctgctatg acaagtcctt ccccatctct cacgtgcgct tctgggtcct
      481 tcagatcata ttcgtgtctg tgcccacact cctgtacttg gcccatgtgt tctatgtgat
      541 gaggaaggaa gagaagctaa acaagaaaga agaggagctc aaagtggccc agactgacgg
      601 ggtcaacgtg gagatgcacc tgaagcagat tgaaatcaag aagttcaagt acgggattga
      661 agagcacggc aaggtgaaaa tgaggggcgg cttgctgaga acctacatca tcagcatcct
      721 cttcaagtct gtcttcgagg tggccttcct gctcatccag tggtacatct atgggttcag
      781 cttgagcgcg gtctacacct gcaagagaga tccctgcccc caccaggtag actgcttcct
      841 ctcacgtccc acggagaaaa ccatcttcat catcttcatg ctggtggtgt ccttggtgtc
      901 tctcgctttg aacatcattg agctcttcta cgtcttcttc aaaggcgtta aggatcgcgt
      961 gaagggaaga agcgatcctt accacgccac cactggccca ctgagcccat caaaagactg
     1021 cggatctcca aaatacgcct acttcaatgg ctgctcctca ccaacggctc cactctcgcc
     1081 tatgtctcct cctgggtaca agctggttac tggtgacaga aacaattcct cgtgccgcaa
     1141 ttacaacaag caagctagcg agcaaaactg ggcgaactac agcgcagagc aaaatcgcat
     1201 ggggcaggcc ggaagcacca tctccaactc gcacgcccag ccgttcgatt tccccgacga
     1261 caaccagaat gccaaaaaag ttgctgctgg acatgaactc cagccattag ccatcgtgga
     1321 ccaacgacct tccagcagag ccagcagccg cgccagcagc aggcctcggc ctgatgacct
     1381 ggagattgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     1441 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1501 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     1561 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     1621 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1681 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1741 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1801 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca gcgacaacgt
     1861 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     1921 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1981 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     2041 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2101 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     2161 accacatt
//
