LOCUS       sequence-85768-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(214..216,217..219,220..933)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(259..280)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(520..539)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     867..888
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctggtaccc ttgcagcttc cagttgtcag
       61 gaagagaagg ctcccatgga tcctgtggtg gtgctggggc tctgtctctc ctgtttgctt
      121 ctcctttcac tctggaaaca gagctatggg ggagggaagc ttcgaattct gcagtcgacg
      181 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      241 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      301 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      361 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      421 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      481 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      541 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      601 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac
      661 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      721 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      781 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      841 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      901 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
      961 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1021 acctgaaaca taaaatgatg cattgttgtt gttacttgtt tatgc
//
