LOCUS       sequence-85775-e        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: GalT Targeting Sequence, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      300..309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(306..308,309..311,312..1025)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(351..372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(612..631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     959..980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcat gaggcttcgg gagccgctcc
       61 tgagcggcag cgccgcgatg ccaggcgcgt ccctacagcg ggcctgccgc ctgctcgtgg
      121 ccgtctgcgc tctgcacctt ggcgtcaccc tcgtttacta cctggctggc cgcgacctga
      181 gccgcctgcc ccaactggtc ggagtctcca caccgctgca gggcggctcg aacagtgccg
      241 ccgccatcgg gcagtcctcc ggggagctcc ggaccggagg ggccaaggat ccaccggtcg
      301 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      361 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      421 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      481 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
      541 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      601 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      661 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      721 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
      781 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
      841 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      901 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
      961 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1021 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     1081 tgctttaaaa acctccc
//
