LOCUS       sequence-85776-e        1389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1389)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-H1-10
FEATURES             Location/Qualifiers
     source          1..1389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      627..636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(633..635,636..638,639..1352)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(678..699)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(939..958)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1286..1307
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agccgccacc atgaccgaga actccacctc cgccccggcg gcgaagccca
       61 aacgggccaa ggcttccaag aagtccacgg accaccccaa gtattcagac atgatcgtgg
      121 ctgctatcca ggcagagaag aaccgtgccg gctcctcgcg ccagtccatc caaaagtata
      181 tcaagagcca ctacaaggtg ggtgagaacg ccgactccca gatcaagttg tccatcaagc
      241 gcctagtgac caccggtgtt ctcaagcaaa ccaaaggggt gggcgcctcg gggtccttca
      301 ggctggccaa gggcgatgag cccaaaaggt cggtggcttt caagaagacc aagaaggaag
      361 tcaagaaagt ggccactcca aagaaggcag ccaagcccaa gaaggctgcc tccaaagccc
      421 caagcaagaa acccaaagcc acccctgtca agaaggccaa gaagaagccg gctgccacgc
      481 ccaagaaagc caaaaagccc aaggttgtca aagtcaaacc agtcaaggcc tccaaaccca
      541 agaaggccaa aaccgtgaag cccaaagcca agtcgagtgc caagaggggg agcaagaaga
      601 agtcgggaag cacggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
      661 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      721 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      781 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      841 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      901 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      961 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1021 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca
     1081 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     1141 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1201 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     1261 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1321 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     1381 ccataccac
//
