LOCUS       sequence-85777-e        1204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1204)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-H2A-10
FEATURES             Location/Qualifiers
     source          1..1204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      439..448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(445..447,448..450,451..1164)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(490..511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(751..770)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1098..1119
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagccgc caccatgtcg ggacgtggca agcagggcgg caaagctcgc
       61 gccaaggcca agacccgctc ttctcgggcc gggcttcagt ttcccgtagg ccgagtgcat
      121 cgcctgctcc gcaaaggcaa ctatgcggag cgggtcggtg ctggagcgcc ggtgtacctg
      181 gcggcggtgc tggagtacct gaccgccgag atcctggagc tggctggcaa cgcggcccgc
      241 gacaacaaga agactcgcat catcccgcgt cacctccagc tggccatccg caacgatgag
      301 gagctcaaca agcttctggg caaagtcacc atcgcacagg gtggcgtcct gcccaacatc
      361 caggccgtgc tactgcccaa gaagaccgag agccaccaca aggcgaaggg caagtcggga
      421 agcacggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      481 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      541 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      601 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      661 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      721 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      781 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      841 gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat
      901 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      961 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1021 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     1081 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1141 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     1201 catt
//
