LOCUS       sequence-85780-e        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-H3-23
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    437..489
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(503..505,506..508,509..1222)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(548..569)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(809..828)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1156..1177
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc tcgtactaaa cagacagctc ggaaatccac
       61 cggcggtaaa gcgccacgca agcagctggc taccaaggct gctcgcaaga gcgcgccggc
      121 taccggcggc gtgaaaaagc ctcaccgtta ccgcccgggc actgtggctc tgcgcgagat
      181 ccgccgctac caaaagtcga ccgagttgct gattcggaag ctgccgttcc agcgcctggt
      241 gcgagaaatc gcccaagact tcaagaccga tcttcgcttc cagagctctg cggtgatggc
      301 gctgcaggag gcttgtgagg cctacttggt agggctcttt gaggacacaa acctttgcgc
      361 catccatgct aagcgagtga ctattatgcc caaagacatc cagctcgctc gccgcattcg
      421 cggagaaaga gcggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      481 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      541 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      601 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      661 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc
      721 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      781 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      841 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      901 ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca acgtctatat
      961 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1021 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1081 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     1141 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1201 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1261 ttgaga
//
