LOCUS       sequence-85781-e        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      498..507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(504..506,507..509,510..1223)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(549..570)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(810..829)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1157..1178
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgcc accatggccc
       61 gaaccaagca gaccgctagg aagtccaccg gtgggaaagc cccccgcaaa cagctggcca
      121 ccaaggcggc tcggaaaagc gcgccctcta ccggcggggt gaagaagcct caccgctaca
      181 ggccagggac cgtggctctg agagagatcc gtcgttacca gaaatcgact gagctgctca
      241 tccggaagct gccattccag agattggtga gggagatcgc ccaggatttc aaaaccgact
      301 tgaggtttca aagtgcagcc atcggtgccc ttcaggaggc tagcgaagca tacctggtgg
      361 ggttgtttga agataccaat ctgtgtgcca tccacgccaa gagagtcacc atcatgccca
      421 aagacatcca gttggctcgc cggatacggg gggagagagc tgggtccgga agcgcaggca
      481 gtggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      541 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      601 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      661 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg
      721 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      781 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      841 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      901 aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata
      961 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1021 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1081 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     1141 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1201 gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca gccatacca
//
