LOCUS       sequence-85783-e        2122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mCerulean-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1363..1365,1366..1368,1369..2082)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1408..1429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1669..1688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2016..2037
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcac catgagcttc accactcgct ccaccttctc caccaactac
       61 cggtccctgg gctctgtcca ggcgcccagc tacggcgccc ggccggtcag cagcgcggcc
      121 agcgtctatg caggcgctgg gggctctggt tcccggatct ccgtgtcccg ctccaccagc
      181 ttcaggggcg gcatggggtc cgggggcctg gccaccggga tagccggggg tctggcagga
      241 atgggaggca tccagaacga gaaggagacc atgcaaagcc tgaacgaccg cctggcctct
      301 tacctggaca gagtgaggag cctggagacc gagaaccgga ggctggagag caaaatccgg
      361 gagcacttgg agaagaaggg accccaggtc agagactgga gccattactt caagatcatc
      421 gaggacctga gggctcagat cttcgcaaat actgtggaca atgcccgcat cgttctgcag
      481 attgacaatg cccgtcttgc tgctgatgac tttagagtca agtatgagac agagctggcc
      541 atgcgccagt ctgtggagaa cgacatccat gggctccgca aggtcattga tgacaccaat
      601 atcacacgac tgcagctgga gacagagatc gaggctctca aggaggagct gctcttcatg
      661 aagaagaacc acgaagagga agtaaaaggc ctacaagccc agattgccag ctctgggttg
      721 accgtggagg tagatgcccc caaatctcag gacctcgcca agatcatggc agacatccgg
      781 gcccaatatg acgagctggc tcggaagaac cgagaggagc tagacaagta ctggtctcag
      841 cagattgagg agagcaccac agtgctcacc acacagtctg ctgaggttgg agctgctgag
      901 acgacgctca cagagctgag acgtacagtc cagtccttgg agatcgacct ggactccatg
      961 agaaatctga aggccagctt ggagaacagc ctgagggagg tggaggcccg ctacgcccta
     1021 cagatggagc agctcaacgg gatcctgctg caccttgagt cagagctggc acagacccgg
     1081 gcagagggac agcgccaggc ccaggagtat gaggccctgc tgaacatcaa ggtcaagctg
     1141 gaggctgaga tcgccaccta ccgccgcctg ctggaagatg gcgaggactt taatcttggt
     1201 gatgccttgg acagcagcaa ctccatgcaa accatccaaa agaccaccac ccgccggata
     1261 gtggatggca aagtggtgtc tgagaccaat gacaccaaag ttctgaggca tcgaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     1381 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1441 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1501 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1561 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     1621 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1681 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1741 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc
     1801 atcagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     1861 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1921 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1981 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2041 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     2101 tcataatcag ccataccaca tt
//
