LOCUS       sequence-85784-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mCerulean-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gatggcgact gcgacccccg tgccgccgcg gatgggcagc cgcgctggcg gccccaccac
      841 gccgctgagc cccacgcgcc tgtcgcggct ccaggagaag gaggagctgc gcgagctcaa
      901 tgaccggctg gcggtgtaca tcgacaaggt gcgcagcctg gagacggaga acagcgcgct
      961 gcagctgcag gtgacggagc gcgaggaggt gcgcggccgt gagctcaccg gcctcaaggc
     1021 gctctacgag accgagctgg ccgacgcgcg acgcgcgctc gacgacacgg cccgcgagcg
     1081 cgccaagctg cagatcgagc tgggcaagtg caaggcggaa cacgaccagc tgctcctcaa
     1141 ctatgctaag aaggaatctg atcttaatgg cgcccagatc aagcttcgag aatatgaagc
     1201 agcactcaat tcgaaagatg cagctcttgc tactgcactt ggtgacaaaa aaagtttaga
     1261 gggagatttg gaggatctga aggatcagat tgcccagttg gaagcctcct tagctgcagc
     1321 caaaaaacag ttagcagatg aaactttact taaagtagat ttggagaatc gttgtcagag
     1381 ccttactgag gacttggagt ttcgcaaaag catgtatgaa gaggagatta acgagaccag
     1441 aaggaagcat gaaacgcgct tggtagaggt ggattctggg cgtcaaattg agtatgagta
     1501 caagctggcg caagcccttc atgagatgag agagcaacat gatgcccaag tgaggctgta
     1561 taaggaggag ctggagcaga cttaccatgc caaacttgag aatgccagac tgtcatcaga
     1621 gatgaatact tctactgtca acagtgccag ggaagaactg atggaaagcc gcatgagaat
     1681 tgagagcctt tcatcccagc tttctaatct acagaaagag tctagagcat gtttggaaag
     1741 gattcaagaa ttagaggact tgcttgctaa agaaaaagac aactctcgtc gcatgctgac
     1801 agacaaagag agagagatgg cggaaataag ggatcaaatg cagcaacagc tgaatgacta
     1861 tgaacagctt cttgatgtaa agttagccct ggacatggaa atcagtgctt acaggaaact
     1921 cttagaaggc gaagaagaga ggttgaagct gtctccaagc ccttcttccc gtgtgacagt
     1981 atcccgagca tcctcaagtc gtagtgtacg tacaactaga ggaaagcgga agagggttga
     2041 tgtggaagaa tcagaggcga gtagtagtgt tagcatctct cattccgcct cagccactgg
     2101 aaatgtttgc atcgaagaaa ttgatgttga tgggaaattt atccgcttga agaacacttc
     2161 tgaacaggat caaccaatgg gaggctggga gatgatcaga aaaattggag acacatcagt
     2221 cagttataaa tatacctcaa gatatgtgct gaaggcaggc cagactgtta caatttgggc
     2281 tgcaaacgct ggtgtcacag ccagcccccc aactgacctc atctggaaga accagaactc
     2341 gtggggcact ggcgaagatg tgaaggttat attgaaaaat tctcagggag aggaggttgc
     2401 tcaaagaagt acagtcttta aaacaaccat acctgaagaa gaggaggagg aggaagaagc
     2461 agctggagtg gttgttgagg aagaactttt ccaccagcag ggaaccccaa gagcatccaa
     2521 tagaagctgt gcaattatgt agggatccac cggatc
//
