LOCUS       sequence-85785-e        1345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1345)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(586..588,589..591,592..1305)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(631..652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(892..911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1239..1260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttat gtcgagcaag
       61 agagccaagg ccaagaccac caagaagagg ccccagaggg ctacgtccaa tgtcttcgcc
      121 atgtttgacc agtcccagat ccaggagttt aaggaggcct tcaacatgat tgatcagaac
      181 cgcgatggct tcattgataa ggaggacctg cacgacatgt tggcctctct ggggaagaac
      241 cccacagacg agtacctgga gggcatgatg aacgaggcgc cagggcccat caacttcacc
      301 atgttcctca caatgtttgg ggagaagctg aacggcacag accccgagga tgtgatccgc
      361 aatgcctttg cctgctttga tgaggaggcc tcaggcttca tccacgagga ccacctgagg
      421 gagctgctca ccaccatggg cgaccgattc acggatgagg aggtggacga gatgtaccgc
      481 gaggcaccca ttgataagaa gggcaacttc aactacgtgg agttcactcg catcctcaaa
      541 cacggcgcca aggacaagga cgacctggat ccaccggtcg ccaccatggt gagcaagggc
      601 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      661 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      721 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      781 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      841 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      901 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      961 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1021 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1081 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1201 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     1321 agatcataat cagccatacc acatt
//
