LOCUS       sequence-85787-e        2047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2047)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctggtggct ccgcaggatc
      781 ccggcaagaa gaagcaaagg ctgctgtagg tgtgactgga aatgacatca ctaccccgcc
      841 aaacaaggag ccaccaccaa gcccagaaaa gaaagcaaag cctttggcca ccactcaacc
      901 tgcaaagact tcaacatcga aagccaaaac acagcccact tctctcccta agcaaccagc
      961 tcccaccacc tctggtgggt tgaataaaaa acccatgagc ctcgcctcag gctcagtgcc
     1021 agctgcccca cacaaacgcc ctgctgctgc cactgctact gccaggcctt ccaccctacc
     1081 tgccagagac gtgaagccaa agccaattac agaagctaag gttgccgaaa agcggacctc
     1141 tccatccaag ccttcatctg ccccagccct caaacctgga cctaaaacca ccccaaccgt
     1201 ttcaaaagcc acatctccct caactcttgt ttccactgga ccaagtagta gaagtccagc
     1261 tacaactctg cctaagaggc caaccagcat caagactgag gggaaacctg ctgatgtcaa
     1321 aaggatgact gctaagtctg cctcagctga cttgagtcgc tcaaagacca cctctgccag
     1381 ttctgtgaag agaaacacca ctcccactgg ggcagcaccc ccagcaggga tgacttccac
     1441 tcgagtcaag cccatgtctg cacctagccg ctcttctggg gctctttctg tggacaagaa
     1501 gcccacttcc actaagccta gctcctctgc tcccagggtg agccgcctgg ccacaactgt
     1561 ttctgcccct gacctgaaga gtgttcgctc caaggtcggc tctacagaaa acatcaaaca
     1621 ccagcctgga ggaggccggg ccaaggtaga gaaaaaaaca gaggcagcta ccacagctgg
     1681 gaagcctgaa cctaatgcag tcactaaagc agccggctcc attgcgagtg cacagaaacc
     1741 gcctgctggg aaagtccaga tagtatccaa aaaagtgagc tacagtcata ttcaatccaa
     1801 gtgtgtttcc aaggacaata ttaagcatgt ccctggatgt ggcaatgttc agattcagaa
     1861 caagaaagtg gacatatcca aggtctcctc caagtgtggg tccaaagcta atatcaagca
     1921 caagcctggt ggaggagatg tcaagattga aagtcagaag ttgaacttca aggagaaggc
     1981 ccaagccaaa gtgggatagg gatccaccgg atctagataa ctgatcataa tcagccatac
     2041 cacattt
//
