LOCUS       sequence-85792-e        1461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1461)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg
      481 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagccatg gctagtcgag
      781 gcgcaacaag acccaacggg ccaaatactg gaaataaaat atgccagttc aaactagtac
      841 ttctgggaga gtctgctgtt ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat
      901 ttcatgaatt tcaagagagt accatagggg ctgcttttct aacccaaact gtgtgtcttg
      961 atgatacaac agtaaagttt gaaatatggg atacagctgg tcaagaacga taccatagct
     1021 tagcaccaat gtactacaga ggagcacaag cagccatagt tgtatatgat atcacaaatg
     1081 aggagtcctt tgccagagcc aaaaactggg ttaaagaact tcagaggcaa gccagtccta
     1141 acattgtaat agctttatca ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt
     1201 tccaggaagc acagtcctat gcagatgaca acagtttatt attcatggag acatcagcta
     1261 aaacatcgat gaatgtaaat gaaatattca tggcaatagc taaaaagttg ccaaagaacg
     1321 aaccacagaa tccaggagca aattctgcca gaggaagagg agtagacctt actgaaccca
     1381 cgcagccaac caggaatcag tgttgtagta actaaggatc caccggatct agataactga
     1441 tcataatcag ccataccaca t
//
