LOCUS       sequence-85795-e        3147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Transferin Receptor, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3147)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-TFR-20
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(986..997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2381..2390
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2387..2389,2390..2392,2393..3106)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2432..2453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2693..2712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3040..3061
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc gccaccatga tggatcaagc
       61 tagatcagca ttctctaact tgtttggtgg agaaccattg tcatataccc ggttcagcct
      121 ggctcggcaa gtagatggcg ataacagtca tgtggagatg aaacttgctg tagatgaaga
      181 agaaaatgct gacaataaca caaaggccaa tgtcacaaaa ccaaaaaggt gtagtggaag
      241 tatctgctat gggactattg ctgtgatcgt ctttttcttg attggattta tgattggcta
      301 cttgggctat tgtaaagggg tagaaccaaa aactgagtgt gagagactgg caggaaccga
      361 gtctccagtg agggaggagc caggagagga cttccctgca gcacgtcgct tatattggga
      421 tgacctgaag agaaagttgt cggagaaact ggacagcaca gacttcacca gcaccatcaa
      481 gctgctgaat gaaaattcat atgtccctcg tgaggctgga tctcaaaaag atgaaaatct
      541 tgcgttgtat gttgaaaatc aatttcgtga atttaaactc agcaaagtct ggcgtgatca
      601 acattttgtt aagattcagg tcaaagacag cgctcaaaac tcggtgatca tagttgataa
      661 gaacggtaga cttgtttacc tggtggagaa tcctgggggt tatgtggcgt atagtaaggc
      721 tgcaacagtt actggtaaac tggtccatgc taattttggt actaaaaaag attttgagga
      781 tttatacact cctgtgaatg gatctatagt gattgtcaga gcagggaaaa tcacctttgc
      841 agaaaaggtt gcaaatgctg aaagcttaaa tgcaattggt gtgttgatat acatggacca
      901 gactaaattt cccattgtta acgcagaact ttcattcttt ggacatgctc atctggggac
      961 aggtgaccct tacacacctg gattcccttc cttcaatcac actcagtttc caccatctcg
     1021 gtcatcagga ttgcctaata tacctgtcca gacaatctcc agagctgctg cagaaaagct
     1081 gtttgggaat atggaaggag actgtccctc tgactggaaa acagactcta catgtaggat
     1141 ggtaacctca gaaagcaaga atgtgaagct cactgtgagc aatgtgctga aagagataaa
     1201 aattcttaac atctttggag ttattaaagg ctttgtagaa ccagatcact atgttgtagt
     1261 tggggcccag agagatgcat ggggccctgg agctgcaaaa tccggtgtag gcacagctct
     1321 cctattgaaa cttgcccaga tgttctcaga tatggtctta aaagatgggt ttcagcccag
     1381 cagaagcatt atctttgcca gttggagtgc tggagacttt ggatcggttg gtgccactga
     1441 atggctagag ggataccttt cgtccctgca tttaaaggct ttcacttata ttaatctgga
     1501 taaagcggtt cttggtacca gcaacttcaa ggtttctgcc agcccactgt tgtatacgct
     1561 tattgagaaa acaatgcaaa atgtgaagca tccggttact gggcaatttc tatatcagga
     1621 cagcaactgg gccagcaaag ttgagaaact cactttagac aatgctgctt tccctttcct
     1681 tgcatattct ggaatcccag cagtttcttt ctgtttttgc gaggacacag attatcctta
     1741 tttgggtacc accatggaca cctataagga actgattgag aggattcctg agttgaacaa
     1801 agtggcacga gcagctgcag aggtcgctgg tcagttcgtg attaaactaa cccatgatgt
     1861 tgaattgaac ctggactatg agaggtacaa cagccaactg ctttcatttg tgagggatct
     1921 gaaccaatac agagcagaca taaaggaaat gggcctgagt ttacagtggc tgtattctgc
     1981 tcgtggagac ttcttccgtg ctacttccag actaacaaca gatttcggga atgctgagaa
     2041 aacagacaga tttgtcatga agaaactcaa tgatcgtgtc atgagagtgg agtatcactt
     2101 cctctctccc tacgtatctc caaaagagtc tcctttccga catgtcttct ggggctccgg
     2161 ctctcacacg ctgccagctt tactggagaa cttgaaactg cgtaaacaaa ataacggtgc
     2221 ttttaatgaa acgctgttca gaaaccagtt ggctctagct acttggacta ttcagggagc
     2281 tgcaaatgcc ctctctggtg acgtttggga cattgacaat gagttttcag aattcggctc
     2341 caccggctcc accggctcca ccggcgcgga tccaccggtc gccaccatgg tgagcaaggg
     2401 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2461 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2521 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2581 gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     2641 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2701 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2761 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2821 cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     2881 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2941 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3001 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3061 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     3121 tagatcataa tcagccatac cacattg
//
