LOCUS       sequence-85796-e        2165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2165)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag cgacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagtgcgtga gtgcatctcc
      781 atccacgttg gccaggctgg tgtccagatt ggcaatgcct gctgggagct ctactgcctg
      841 gaacacggca tccagcccga tggccagatg ccaagtgaca agaccattgg gggaggagat
      901 gactccttca acaccttctt cagtgagacg ggcgctggca agcacgtgcc ccgggctgtg
      961 tttgtagact tggaacccac agtcattgat gaagttcgca ctggcaccta ccgccagctc
     1021 ttccaccctg agcagctcat cacaggcaag gaagatgctg ccaataacta tgcccgaggg
     1081 cactacacca ttggcaagga gatcattgac cttgtgttgg accgaattcg caagctggct
     1141 gaccagtgca ccggtcttca gggcttcttg gttttccaca gctttggtgg gggaactggt
     1201 tctgggttca cctccctgct catggaacgt ctctcagttg attatggcaa gaagtccaag
     1261 ctggagttct ccatttaccc agcaccccag gtttccacag ctgtagttga gccctacaac
     1321 tccatcctca ccacccacac caccctggag cactctgatt gtgccttcat ggtagacaat
     1381 gaggccatct atgacatctg tcgtagaaac ctcgatatcg agcgcccaac ctacactaac
     1441 cttaaccgcc ttattagcca gattgtgtcc tccatcactg cttccctgag atttgatgga
     1501 gccctgaatg ttgacctgac agaattccag accaacctgg tgccctaccc ccgcatccac
     1561 ttccctctgg ccacatatgc ccctgtcatc tctgctgaga aagcctacca tgaacagctt
     1621 tctgtagcag agatcaccaa tgcttgcttt gagccagcca accagatggt gaaatgtgac
     1681 cctcgccatg gtaaatacat ggcttgctgc ctgttgtacc gtggtgacgt ggttcccaaa
     1741 gatgtcaatg ctgccattgc caccatcaaa accaagcgca gcatccagtt tgtggattgg
     1801 tgccccactg gcttcaaggt tggcatcaac taccagcctc ccactgtggt gcctggtgga
     1861 gacctggcca aggtacagag agctgtgtgc atgctgagca acaccacagc cattgctgag
     1921 gcctgggctc gcctggacca caagtttgac ctgatgtatg ccaagcgtgc ctttgttcac
     1981 tggtacgtgg gtgaggggat ggaggaaggc gagttttcag aggcccgtga agatatggct
     2041 gcccttgaga aggattatga ggaggttggt gtggattctg ttgaaggaga gggtgaggaa
     2101 gaaggagagg aatactaagg atccaccgga tctagataac tgatcataat cagccatacc
     2161 acatt
//
