LOCUS       sequence-85802-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER Transport, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-VSVG-16
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(220..222,223..225,226..939)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(265..286)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(526..545)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     873..894
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggttggttca gtagttggaa gagctctatt gcctcttttt tctttatcat agggttaatc
       61 attggactat tcttggttct ccgagttggt atttatcttt gcattaaatt aaagcacacc
      121 aagaaaagac agatttatac agacatagag atgaaccgac ttggaaacag aattctgcag
      181 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
      241 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      301 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      361 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
      421 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      481 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      541 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      601 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
      661 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
      721 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      781 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
      841 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      901 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
      961 taatcagcca taccacat
//
