LOCUS       sequence-85803-e        2539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mCerulean-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1774..1783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1780..1782,1783..1785,1786..2499)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1825..1846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2086..2105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2433..2454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc
       61 ccgtctcccg cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag
      121 ttcggccctg tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag
      181 cctcccccgg cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg
      241 ccgcccccgg aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca
      301 gagggtgctc tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc
      361 cctgcgcctc tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga
      421 gggcctgagg cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat
      481 ttggagatcg actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa
      541 gcccgggtgt catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc
      601 agtaccaagc ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc
      661 tcccagcctc tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag
      721 ccccagcccc agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg
      781 tctttggcta acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag
      841 ttttctccag tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca
      901 ggtggatctg ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc
      961 acaggctccc ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg
     1021 caggagaagc agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct
     1081 ggggccccag ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag
     1141 ctaatgcagg acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc
     1201 cgatgccatc aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc
     1261 cacatcgcct gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac
     1321 agtctggagg gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac
     1381 acctgcgggg agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg
     1441 cactgcttca cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac
     1501 caggccaacc ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc
     1561 gtctgctctg agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc
     1621 ctggacaaga acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg
     1681 attgaggcag atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc
     1741 cacactgcta gagcccagac ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     1801 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1861 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1921 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1981 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2041 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2101 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2161 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
     2221 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     2281 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2341 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     2401 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2461 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     2521 taatcagcca taccacatt
//
