LOCUS       sequence-85805-e        1980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtgacgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgagctgggg
      241 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcca
      481 cggcaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcggcct
      541 caactgcaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg
      781 aggcagcgca tccggcggaa gcggaagcga tgatgatatc gccgcgctcg tcgtcgacaa
      841 cggctccggc atgtgcaagg ccggcttcgc gggcgacgat gccccccggg ccgtcttccc
      901 ctccatcgtg gggcgcccca ggcaccaggg cgtgatggtg ggcatgggtc agaaggattc
      961 ctatgtgggc gacgaggccc agagcaagag aggcatcctc accctgaagt accccatcga
     1021 gcacggcatc gtcaccaact gggacgacat ggagaaaatc tggcaccaca ccttctacaa
     1081 tgagctgcgt gtggctcccg aggagcaccc cgtgctgctg accgaggccc ccctgaaccc
     1141 caaggccaac cgcgagaaga tgacccagat catgtttgag accttcaaca ccccagccat
     1201 gtacgttgct atccaggctg tgctatccct gtacgcctct ggccgtacca ctggcatcgt
     1261 gatggactcc ggtgacgggg tcacccacac tgtgcccatc tacgaggggt atgccctccc
     1321 ccatgccatc ctgcgtctgg acctggctgg ccgggacctg actgactacc tcatgaagat
     1381 cctcaccgag cgcggctaca gcttcaccac cacggccgag cgggaaatcg tgcgtgacat
     1441 taaggagaag ctgtgctacg tcgccctgga cttcgagcaa gagatggcca cggctgcttc
     1501 cagctcctcc ctggagaaga gctacgagct gcctgacggc caggtcatca ccattggcaa
     1561 tgagcggttc cgctgccctg aggcactctt ccagccttcc ttcctgggca tggagtcctg
     1621 tggcatccac gaaactacct tcaactccat catgaagtgt gacgtggaca tccgcaaaga
     1681 cctgtacgcc aacacagtgc tgtctggcgg caccaccatg taccctggca ttgccgacag
     1741 gatgcagaag gagatcactg ccctggcacc cagcacaatg aagatcaaga tcattgctcc
     1801 tcctgagcgc aagtactccg tgtggatcgg cggctccatc ctggcctcgc tgtccacctt
     1861 ccagcagatg tggatcagca agcaggagta tgacgagtcc ggcccctcca tcgtccaccg
     1921 caaatgcttc tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
